裴炜炜, 陶立静, 聂继华, 童建. 氡暴露致小鼠肺组织线粒体转录因子A及核因子-κB表达的改变[J]. 环境与职业医学, 2016, 33(4): 385-389. DOI: 10.13213/j.cnki.jeom.2016.15434
引用本文: 裴炜炜, 陶立静, 聂继华, 童建. 氡暴露致小鼠肺组织线粒体转录因子A及核因子-κB表达的改变[J]. 环境与职业医学, 2016, 33(4): 385-389. DOI: 10.13213/j.cnki.jeom.2016.15434
PEI Wei-wei, TAO Li-jing, NIE Ji-hua, TONG Jian. Alteration in Expression of Mitochondrial Transcription Factor A and Nuclear Factor-κB in Mouse Lung Tissue Exposed to Radon[J]. Journal of Environmental and Occupational Medicine, 2016, 33(4): 385-389. DOI: 10.13213/j.cnki.jeom.2016.15434
Citation: PEI Wei-wei, TAO Li-jing, NIE Ji-hua, TONG Jian. Alteration in Expression of Mitochondrial Transcription Factor A and Nuclear Factor-κB in Mouse Lung Tissue Exposed to Radon[J]. Journal of Environmental and Occupational Medicine, 2016, 33(4): 385-389. DOI: 10.13213/j.cnki.jeom.2016.15434

氡暴露致小鼠肺组织线粒体转录因子A及核因子-κB表达的改变

Alteration in Expression of Mitochondrial Transcription Factor A and Nuclear Factor-κB in Mouse Lung Tissue Exposed to Radon

  • 摘要: 目的

    观察氡对小鼠肺组织线粒体转录因子A(TFAM)及核因子(NF)-κB表达的影响,为探讨氡致肺损伤的机制提供实验依据。

    方法

    20只健康雌性BALB/c小鼠随机分为对照组及3个染氡组,每组5只,染氡组氡暴露剂量分别为30、60、120工作水平月。末次染毒后1 h内处死小鼠,取肺组织进行HE染色以及TFAM和NF-κB p65免疫组化染色,Western blot检测肺组织中TFAM和NF-κB p65的蛋白表达。

    结果

    免疫组化结果显示,染氡后TFAM、NF-κB p65阳性细胞数显著增加,其中120工作水平月组的TFAM和NF-κB p65阳性细胞率(0.521±0.081)%、(0.260±0.068)%高于对照组(0.093±0.011)%、(0.069±0.016)%,P < 0.05,Western blot结果显示其蛋白表达也显著上升。

    结论

    氡暴露小鼠肺组织中TFAM和NF-κB的表达上调,提示NF-κB的信号通路被激活。

     

    Abstract: Objective

    To observe changes in expression of mitochondrial transcription factor A (TFAM) and nuclear factor kappa B (NF-κB) in mouse lung tissue induced by radon, so as to provide experimental evidence for understanding related mechanism.

    Methods

    Twenty healthy female BALB/c mice were randomly divided into one control group and three experimental groups with cumulative doses of radon at 30, 60, and 120 working level months (WLM). All mice were sacrificed 1 h after last treatment. The lung tissues were stained with HE, and the expressions of TFAM and NF-κB p65 were detected by im munohistochemistry and Western blot.

    Results

    The immunohistochemistry results showed that the positive cell rates of TFAM and NF-κB p65 significantly increased after radon exposure, and the 120 WLM group presented higher positive cell rates of TFAM and NF-κB p65 (0.521±0.081)%, (0.260±0.068)% than the control group (0.093±0.011)%, (0.069±0.016)%, P < 0.05. So did the expression of protein according to Western blot.

    Conclusion

    The elevated expressions of TFAM and NF-κB induced by radon indicate an activation of NF-κB signaling pathway.

     

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