何启娜, 吉永新, 孙玉龙, 曹毅. 活性氧在900 MHz微波辐射诱导小鼠骨髓基质细胞适应性反应中的作用[J]. 环境与职业医学, 2016, 33(2): 123-128. DOI: 10.13213/j.cnki.jeom.2016.15394
引用本文: 何启娜, 吉永新, 孙玉龙, 曹毅. 活性氧在900 MHz微波辐射诱导小鼠骨髓基质细胞适应性反应中的作用[J]. 环境与职业医学, 2016, 33(2): 123-128. DOI: 10.13213/j.cnki.jeom.2016.15394
HE Qi-na, JI Yong-xin, SUN Yu-long, CAO Yi. Role of Reactive Oxygen Species in Adaptive Response Induced by 900 MHz Radiofrequency Field in Mouse Bone Marrow Stromal Cells[J]. Journal of Environmental and Occupational Medicine, 2016, 33(2): 123-128. DOI: 10.13213/j.cnki.jeom.2016.15394
Citation: HE Qi-na, JI Yong-xin, SUN Yu-long, CAO Yi. Role of Reactive Oxygen Species in Adaptive Response Induced by 900 MHz Radiofrequency Field in Mouse Bone Marrow Stromal Cells[J]. Journal of Environmental and Occupational Medicine, 2016, 33(2): 123-128. DOI: 10.13213/j.cnki.jeom.2016.15394

活性氧在900 MHz微波辐射诱导小鼠骨髓基质细胞适应性反应中的作用

Role of Reactive Oxygen Species in Adaptive Response Induced by 900 MHz Radiofrequency Field in Mouse Bone Marrow Stromal Cells

  • 摘要: 目的

    探讨活性氧(ROS)在900 MHz微波辐射诱导γ射线致小鼠骨髓基质细胞DNA损伤的适应性反应中的作用。

    方法

    给予小鼠骨髓基质细胞120 μW/cm2,900 MHz微波照射,4 h/d,连续5 d,微波照射结束4 h后给予1.5 Gy γ射线照射,照射结束后立即采用碱性单细胞凝胶电泳试验和γ-H2AX焦点形成试验检测DNA损伤,观察微波辐射诱导的适应性反应;采用500 nmol/L褪黑素(ROS抑制剂)在微波照射前4 h处理细胞,流式细胞仪检测ROS水平的变化,观察适应性反应的变化情况。

    结果

    微波照射后细胞内ROS水平(2.10)较对照组(1.16)升高(P < 0.01)。与单独给予γ射线照射的彗星尾长、尾矩和γ-H2AX焦点形成数(分别为21.62 μm、5.68、19.73)相比,预先给予微波照射可明显减轻随后γ射线照射造成的DNA损伤,彗星尾长、尾矩和γ-H2AX焦点形成数(分别为8.57 μm、2.58、5.28)明显减少(P < 0.001),诱导出适应性反应。褪黑素预处理细胞后,微波诱导产生的ROS下降为1.53(P < 0.01),微波拮抗γ射线照射造成的DNA损伤程度也随之下降,彗星尾长、尾矩和γ-H2AX焦点形成数(分别为17.90 μm、4.10、20.8)明显升高(P < 0.001),适应性反应受到抑制。

    结论

    ROS可能在900 MHz微波辐射诱导细胞适应性反应中发挥作用。

     

    Abstract: Objective

    To study the role of reactive oxygen species (ROS) in adaptive response induced by 900 MHz microwave radiation in mouse bone marrow stromal cells (BMSCs) exposed to γ rays.

    Methods

    BMSCs were pre-exposed to 900 MHz radiofrequency fields (RF) at 120 μW/cm2 power intensity for 4 h/d for 5 d and then subjected to an acute dose of 1.5 Gy γ-ray radiation. The level of DNA strand breaks was assessed by alkaline comet assay and γ-H2AX foci technique to confirm adaptive response induced by microwave radiation. The cells were exposed to 500 nmol/L melatonin (an inhibitor for ROS) 4 h prior to the RF exposure to measure the level of ROS using flow cytometer and confirm adaptive response.

    Results

    The cells exposed to RF showed a significantly increase of ROS (2.10) as compared to the control group (1.16) (P < 0.01). The cells preexposed to RF showed significant decreased DNA damage as well as reduced tail length, tail moment, and γ-H2AX foci (8.57 μm, 2.58, and 5.28, respectively) compared with those exposed to γ-radiation alone (21.62 μm, 5.68, and 19.73, respectively) (P < 0.001), indicating that RF induced adaptive response. However, when the cells were subjected to melatonin prior to RF, the ROS level was decreased to 1.53 (P < 0.01), and the DNA damage induced by subsequent γ-ray radiation decreased significantly as the tail length, tail moment, and γ-H2AX foci were increased (17.90 μm, 4.10, and 20.8, respectively) (P < 0.001), indicating that adaptive response was inhibited.

    Conclusion

    ROS might play a role in the adaptive response induced by 900 MHz radiofrequency field at 120 μW/cm2 power density.

     

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