聂琛, 关怀, 李秋娟, 李双月, 张艳, 冯丽娜, 郝捷, 袁利刚. 孕期尼古丁暴露对子代大鼠脑组织单胺类神经递质水平的影响[J]. 环境与职业医学, 2016, 33(1): 53-56. DOI: 10.13213/j.cnki.jeom.2016.15364
引用本文: 聂琛, 关怀, 李秋娟, 李双月, 张艳, 冯丽娜, 郝捷, 袁利刚. 孕期尼古丁暴露对子代大鼠脑组织单胺类神经递质水平的影响[J]. 环境与职业医学, 2016, 33(1): 53-56. DOI: 10.13213/j.cnki.jeom.2016.15364
NIE Chen, GUAN Huai, LI Qiu-juan, LI Shuang-yue, ZHANG Yan, FENG Li-na, HAO Jie, YUAN Li-gang. Effects of Gestational Exposure to Nicotine on Levels of Monoamine Neurotransmitters in Brain Tissues of Rat Offspring[J]. Journal of Environmental and Occupational Medicine, 2016, 33(1): 53-56. DOI: 10.13213/j.cnki.jeom.2016.15364
Citation: NIE Chen, GUAN Huai, LI Qiu-juan, LI Shuang-yue, ZHANG Yan, FENG Li-na, HAO Jie, YUAN Li-gang. Effects of Gestational Exposure to Nicotine on Levels of Monoamine Neurotransmitters in Brain Tissues of Rat Offspring[J]. Journal of Environmental and Occupational Medicine, 2016, 33(1): 53-56. DOI: 10.13213/j.cnki.jeom.2016.15364

孕期尼古丁暴露对子代大鼠脑组织单胺类神经递质水平的影响

Effects of Gestational Exposure to Nicotine on Levels of Monoamine Neurotransmitters in Brain Tissues of Rat Offspring

  • 摘要: 目的

    探讨孕期尼古丁暴露对子代大鼠脑组织单胺类神经递质水平的影响。

    方法

    24只SD受孕大鼠,随机分为4组,即对照组和1.0、2.0、4.0 mg/kg染毒组,每组6只。孕7天起,对照组孕鼠皮下注射生理盐水1 mL,染毒组孕鼠皮下注射尼古丁生理盐水溶液1 mL(各染毒组按1.0、2.0、4.0 mg/kg计算尼古丁量),1次/d,直至分娩。测定5周龄子鼠体重、脑重,计算脑脏器系数;测定5周龄子鼠大脑皮质和海马中去甲肾上腺素(NE)、多巴胺(DA)和五羟色胺(5-HT)的质量分数(后简称“含量”)。

    结果

    4.0 mg/kg染毒组子鼠脑重和脑脏器系数低于另3组(P < 0.05)。3个染毒组子鼠大脑皮层NE、DA和5-HT含量均低于对照组(P < 0.05);4.0 mg/kg染毒组子鼠NE含量低于另2个染毒组(P < 0.05),2.0、4.0 mg/kg染毒组子鼠DA含量低于1.0 mg/kg染毒组(P < 0.05),4.0 mg/kg染毒组子鼠5-HT含量低于1.0 mg/kg染毒组(P < 0.05)。3个染毒组子鼠海马NE和DA含量均低于对照组(P < 0.05);海马DA含量与染毒剂量间呈剂量-效应关系(P < 0.05),4.0 mg/kg染毒组子鼠海马5-HT含量低于对照组(P < 0.05)。

    结论

    孕期尼古丁暴露影响子代大脑皮层和海马单胺类神经递质含量,可能参与其导致的神经发育毒性过程。

     

    Abstract: Objective

    To evaluate the effects of gestational exposure to nicotine on the levels of monoamine neurotransmitters in various brain regions of rat offspring.

    Methods

    Twenty-four pregnant SD rats were randomly divided into four groups and treated with nicotine saline at dose of 0.0, 1.0, 2.0, and 4.0 mg/kg daily through subcutaneous injection starting from the seventh gestational day until delivery. At the age of 5 weeks, the body and brain weights of offspring were measured, brain organ coefficient was calculated, and the levels of norepinephrine (NE), dopamine (DA), and 5-hydroxytryptamine (5-HT) in cerebral cortex and hippocampus of offspring were detected.

    Results

    The brain weight and brain organ coefficient of the 4.0 mg/kg nicotine treatment group were significantly lower than those of the other three groups (P < 0.05). In offspring cerebral cortex, the levels of NE, DA, and 5-HT of the three nicotine exposed groups were significantly lower than those of the control group (P < 0.05); the level of NE of the 4.0 mg/kg nicotine treatment group was significantly lower than those of the other two nicotine exposed groups (P < 0.05); the levels of DA of the 2.0 mg/kg and 4.0 mg/kg nicotine exposed groups were significantly lower than that of the 1.0 mg/kg nicotine exposed group (P < 0.05); the level of 5-HT of the 4.0 mg/kg nicotine exposed group was significantly lower than that of the 1.0 mg/kg nicotine exposed group (P < 0.05). In offspring hippocampus, the levels of NE and DA of the three nicotine exposed groups were significantly lower than those of the control group (P < 0.05); a dose-effect relationship was found between the DA level and the dose of nicotine treatment (P < 0.05); the level of 5-HT in the 4.0 mg/kg nicotine exposed group was significantly lower than those of the control group (P < 0.05).

    Conclusion

    Gestational exposure to nicotine could influence the levels of monoamine neurotransmitters in the cerebral cortex and hippocampus of rat offspring, which might participate the neurodevelopmental toxicity induced by nicotine.

     

/

返回文章
返回