孙立萍, 王克跃, 李红. 亚慢性钒染毒对大鼠脑生长发育及细胞凋亡的影响[J]. 环境与职业医学, 2015, 32(12): 1166-1170. DOI: 10.13213/j.cnki.jeom.2015.15230
引用本文: 孙立萍, 王克跃, 李红. 亚慢性钒染毒对大鼠脑生长发育及细胞凋亡的影响[J]. 环境与职业医学, 2015, 32(12): 1166-1170. DOI: 10.13213/j.cnki.jeom.2015.15230
SUN Li-ping , WANG Ke-yue , LI Hong . Effects of Subchronic Exposure to Vanadium on Brain Development and Cell Apoptosis of Rats[J]. Journal of Environmental and Occupational Medicine, 2015, 32(12): 1166-1170. DOI: 10.13213/j.cnki.jeom.2015.15230
Citation: SUN Li-ping , WANG Ke-yue , LI Hong . Effects of Subchronic Exposure to Vanadium on Brain Development and Cell Apoptosis of Rats[J]. Journal of Environmental and Occupational Medicine, 2015, 32(12): 1166-1170. DOI: 10.13213/j.cnki.jeom.2015.15230

亚慢性钒染毒对大鼠脑生长发育及细胞凋亡的影响

Effects of Subchronic Exposure to Vanadium on Brain Development and Cell Apoptosis of Rats

  • 摘要: 目的 观察亚慢性染毒偏钒酸钠对大鼠脑生长发育及海马、皮质、纹状体的细胞凋亡的影响。

    方法 21 日龄健康雄性SD大鼠49 只,随机分为对照组(n=12)及低(n=12)、中(n=12)、高(n=13)染钒组偏钒酸钠(NaVO3)0.5、1.0、2.0 mg/mL。饮水染毒3 个月后,取脑测脏器系数,并观察海马、皮质和纹状体组织学变化。流式细胞仪检测海马、皮质和纹状体的细胞凋亡率。

    结果 低钒组(0.466& #177;0.077)、中钒组(0.470& #177;0.058)、高钒组(0.712& #177;0.262)的大鼠脑脏器系数高于对照组(0.392& #177;0.023)(P<0.05),且高钒组高于低、中染钒组(P<0.05)。HE 染色显示,染钒组海马、皮质、纹状体均出现组织疏松、水肿和毛细血管扩张,部分可见神经细胞嗜酸性变,神经元凋亡及噬神经现象。在海马组织,中染钒组(7.45%,7.55%)、高染钒组(14.30%,15.00%)的早期凋亡率和总凋亡率高于对照组(1.65%,1.75%),高染钒组高于低染钒组(4.10%,4.10%)(P<0.01);在纹状体组织,中染钒组(9.50%,9.80%)、高染钒组(13.55%,14.65%)的早期凋亡率和总凋亡率高于对照组(2.15%,2.35%)(P<0.01);在皮质组织,中染钒组(10.65%,11.10%)、高染钒组(16.75%,17.40%)早期凋亡率和总凋亡率高于对照组(3.15%,3.15%),高钒组高于低钒组(8.30%,8.55%)(P<0.01),中染钒组(0.45%)、高染钒组(0.50%)晚期凋亡率高于对照组(P<0.01)。

    结论 亚慢性染钒可导致海马、皮质、纹状体神经细胞凋亡,影响大鼠脑生长发育。

     

    Abstract: Objective To observe the effects of subchronic sodium metavanadate exposure on brain development and cell apoptosis in hippocampus, cortex, and striatum of rats.

    Methods Healthy male SD rats (n=49) weaned at 21 d of age were randomly divided into one blank control and three vanadium groups 0.5, 1.0, and 2.0 mg/mL sodium metavanadate (NaVO3). After administration via drinking water for 3 months, necroscopy was conducted to determine the weight coefficient of brain and observe the histological changes in hippocampus, cortex, and striatum. Cell apoptosis in hippocampus, cortex, and striatum was detected by flow cytometry.

    Results The organ coefficients of brain for the 0.5 mg/mL vanadium group (0.466& #177;0.077), 1.0 mg/mL vanadium group (0.470& #177;0.058), and 2.0 mg/mL vanadium group (0.712& #177;0.262) were higher than that in the control group (0.392& #177; 0.023) (P<0.05), and the 2.0 mg/mL vanadium group presented higher values than the 0.5 mg/mL and 1.0 mg/mL vanadium groups (P<0.05). HE staining showed tissue loose, edema, and capillary expansion in the hippocampus, cortex, and striatum of vanadium treated groups, as well as scattered eosinophilic changes, neuron apoptosis, and neuronophagia. In hippocampus, the early apoptosis rates and the total apoptosis rates for the 1.0 mg/mL vanadium group (7.45%, 7.55%) and 2.0 mg/mL vanadium group (14.30%, 15.00%) were higher than those of the control group (1.65%, 1.75%), and the 2.0 mg/mL vanadium group also presented higher values than the 0.5 mg/mL vanadium group (4.10%, 4.10%) (P<0.01). In striatum, the early apoptosis rates and the total apoptosis rates for the 1.0 mg/mL vanadium group (9.50%, 9.80%) and 2.0 mg/mL vanadium group (13.55%, 14.65%) were higher than those of the control group (2.15%, 2.35%) (P<0.01). In cortex, the early apoptosis rates and the total apoptosis rates for the 1.0 mg/mL vanadium group (10.65%, 11.10%) and 2.0 mg/mL vanadium group (16.75%, 17.40%) were higher than those of the control group (3.15%, 3.15%), those of the 2.0 mg/mL vanadium group were higher than those of the 1.0 mg/mL vanadium group (8.30%, 8.55%) (P<0.01), and late apoptosis showed similar patterns that the 1.0 mg/mL (0.45%) and 2.0 mg/mL (0.50%) groups reported higher rates than the control group (P<0.01).

    Conclusion Subchronic exposure to vanadium leads to cell apoptosis in hippocampus, cortex, and striatum of rats, affecting the development of brain.

     

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