高慧艳, 李江敏, 王燕, 陈小玉. 氯化汞对睾丸间质细胞类固醇激素合成影响的体外研究[J]. 环境与职业医学, 2015, 32(10): 970-973,978. DOI: 10.13213/j.cnki.jeom.2015.15206
引用本文: 高慧艳, 李江敏, 王燕, 陈小玉. 氯化汞对睾丸间质细胞类固醇激素合成影响的体外研究[J]. 环境与职业医学, 2015, 32(10): 970-973,978. DOI: 10.13213/j.cnki.jeom.2015.15206
GAO Hui-yan , LI Jiangmin , WANG Yan , CHEN Xiao-yu . In Vitro Effects of Mercury on Steroid Hormone Biosynthesis of Leydig Cells[J]. Journal of Environmental and Occupational Medicine, 2015, 32(10): 970-973,978. DOI: 10.13213/j.cnki.jeom.2015.15206
Citation: GAO Hui-yan , LI Jiangmin , WANG Yan , CHEN Xiao-yu . In Vitro Effects of Mercury on Steroid Hormone Biosynthesis of Leydig Cells[J]. Journal of Environmental and Occupational Medicine, 2015, 32(10): 970-973,978. DOI: 10.13213/j.cnki.jeom.2015.15206

氯化汞对睾丸间质细胞类固醇激素合成影响的体外研究

In Vitro Effects of Mercury on Steroid Hormone Biosynthesis of Leydig Cells

  • 摘要: 目的 探讨氯化汞(HgCl2)对SD大鼠体外培养睾丸间质细胞(leydig cell)类固醇激素合成的影响。

    方法 不同浓度HgCl2 (1& #215;10-8、1& #215;10-7、1& #215;10-6、1& #215;10-5 mol/L)作用于leydig 细胞后, 测定孕酮含量, 运用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术检测HgCl2 对leydig 细胞中StAR、P450scc 和3β-HSD mRNA表达的影响。

    结果 经不同浓度HgCl2 染毒后, leydig 细胞分泌孕酮的量均有所降低, 其中1& #215;10-7、1& #215;10-6、1& #215;10-5 mol/L HgCl2 染毒组与对照组相比, 差异均有统计学意义(P < 0.05)。1& #215;10-7、1& #215;10-6 和1& #215;10-5 mol/L HgCl2 剂量组的StAR、P450scc 和3β-HSD mRNA相对表达量均低于对照组(P < 0.05)。

    结论 汞可以影响睾丸间质细胞类固醇激素的合成。

     

    Abstract: Objective To assess the effects of mercury chloride (HgCl2) on steroid hormone biosynthesis in leydig cells isolated from SD rat testis.

    Methods Leydig cells were exposed to various concentrations of HgCl2 (1& #215;10-8, 1& #215;10-7, 1& #215;10-6, and 1& #215;10-5 mol/L). Progesterone in the supernatant was measured. The levels of mRNA expression of StAR, P450scc, and 3β-HSD in leydig cells were determined by real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR).

    Results The progesterone content secreted by Leydis cells was reduced after treated by various concentrations of HgCl2, and the progesterone contents in the 1& #215;10-7, 1& #215;10-6, and 1& #215;10-5 mol/L HgCl2 groups were significantly lower than that of the control group (P < 0.05). The mRNA relative expression levels of StAR, P450scc, and 3β-HSD in the leydig cells exposed to HgCl2 at the concentration of 1& #215;10-7, 1& #215;10-6, and 1& #215;10-5 mol/L were also statistically lower than that of the control group (P < 0.05).

    Conclusion Mercury could affect steroid hormone biosynthesis of leydig cells.

     

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