王新金, 常秀丽, 周志俊. 甲基汞通过miRNA对人胚胎神经干细胞细胞周期相关基因表达的调控[J]. 环境与职业医学, 2015, 32(5): 455-459. DOI: 10.13213/j.cnki.jeom.2015.14788
引用本文: 王新金, 常秀丽, 周志俊. 甲基汞通过miRNA对人胚胎神经干细胞细胞周期相关基因表达的调控[J]. 环境与职业医学, 2015, 32(5): 455-459. DOI: 10.13213/j.cnki.jeom.2015.14788
WANG Xin-jin , CHANG Xiu-li , ZHOU Zhi-jun . Methylmercury Tunes Cell Cycle Regulated Gene Expressions in Human Embryonic Neural Stem Cells through miRNAs[J]. Journal of Environmental and Occupational Medicine, 2015, 32(5): 455-459. DOI: 10.13213/j.cnki.jeom.2015.14788
Citation: WANG Xin-jin , CHANG Xiu-li , ZHOU Zhi-jun . Methylmercury Tunes Cell Cycle Regulated Gene Expressions in Human Embryonic Neural Stem Cells through miRNAs[J]. Journal of Environmental and Occupational Medicine, 2015, 32(5): 455-459. DOI: 10.13213/j.cnki.jeom.2015.14788

甲基汞通过miRNA对人胚胎神经干细胞细胞周期相关基因表达的调控

Methylmercury Tunes Cell Cycle Regulated Gene Expressions in Human Embryonic Neural Stem Cells through miRNAs

  • 摘要: 目的 通过研究甲基汞对人胚胎神经干细胞miRNA表达的影响,探讨低剂量甲基汞对神经干细胞细胞周期调控基因的调节作用。

    方法 以0、10、50 nmol/L甲基汞染毒人胚胎神经干细胞24 h后,用MTT法测定甲基汞对细胞活力影响,应用逆转录多聚酶链反应(RT-PCR)检测甲基汞对细胞周期调控基因(p16p21)的mRNA的表达水平影响,利用实时荧光定量多聚酶链反应技术检测调控p16p21的miRNA (miR-24、miR-106a)的表达情况。

    结果 50 nmol/L甲基汞染毒组细胞活力降低为对照组的53.5%,差异有统计学意义(P < 0.05);p16p21基因的mRNA表达水平随着甲基汞染毒浓度的升高而升高,差异均有统计学意义(P < 0.05),但10 nmol/L与50 nmol/L组的p16基因表达差异无统计学意义。miR-24、miR-106a的表达水平随着甲基汞染毒浓度的升高而降低,差异有统计学意义(P < 0.05)。

    结论 50 nmol/L的甲基汞可以引起人胚胎神经干细胞增殖抑制,并可能通过miRNA调节细胞周期调控基因的表达。

     

    Abstract: Objective To investigate the impact on alteration of cell cycle regulated genes induced by low-level methylmercury via the expression of microRNAs of human embryonic neural stem cells.

    Methods After treatment with 0,10,and 50 nmol/L methylmercury for 24 h to human embryonic neural stem cells,cell viability was measured with methyl thiazolyl tetrazolium (MTT) assay.mRNA expressions of cell cycle regulated genes p16 and p21 were determined in human embryonic stem cells after the methylmercury exposure by reverse transcription polymerase chain reaction (RT-PCR).Expressions of miRNA (miR-24 and miR-106a) were determined using quantitative real time polymerase chain reaction (qRT-PCR) assay.

    Results Compared with the control group,the cell viability was significantly reduced by 53.5% in the 50 nmol/L methylmercury treatment group (P < 0.05).Compared with the control group,RT-PCR analysis revealed significant methylmercury-induced up-regulations of p16 and p21 mRNA expressions (P < 0.05),but no difference in p16 expression level was found between the 10 nmol/L and the 50 nmol/L treatment.The expressions of miR-24 and miR-106a were significantly lower with the increases in methylmercury concentrations (P < 0.05).

    Conclusion Methylmercury treatment at 50 nmol/L could restrain the self-renewal ability of neural stem cells and tune cell cycle regulators transcription through altering the expression of miRNAs.

     

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