于茂辉, 刁海鹏, 冀晓丽, 刘庆平, 周志俊, 吴庆. 苯并[a]芘对小鼠睾丸支持细胞缝隙连接蛋白表达及细胞增殖的影响[J]. 环境与职业医学, 2015, 32(5): 471-475. DOI: 10.13213/j.cnki.jeom.2015.14767
引用本文: 于茂辉, 刁海鹏, 冀晓丽, 刘庆平, 周志俊, 吴庆. 苯并[a]芘对小鼠睾丸支持细胞缝隙连接蛋白表达及细胞增殖的影响[J]. 环境与职业医学, 2015, 32(5): 471-475. DOI: 10.13213/j.cnki.jeom.2015.14767
YU Mao-hui , DIAO Hai-peng , JI Xiao-li , LIU Qing-ping , ZHOU Zhi-jun , WU Qing . Effects of Benzo[a]pyrene on Expression of Junction Protein and Proliferation in Mouse Sertoli Cells[J]. Journal of Environmental and Occupational Medicine, 2015, 32(5): 471-475. DOI: 10.13213/j.cnki.jeom.2015.14767
Citation: YU Mao-hui , DIAO Hai-peng , JI Xiao-li , LIU Qing-ping , ZHOU Zhi-jun , WU Qing . Effects of Benzo[a]pyrene on Expression of Junction Protein and Proliferation in Mouse Sertoli Cells[J]. Journal of Environmental and Occupational Medicine, 2015, 32(5): 471-475. DOI: 10.13213/j.cnki.jeom.2015.14767

苯并a芘对小鼠睾丸支持细胞缝隙连接蛋白表达及细胞增殖的影响

Effects of Benzoapyrene on Expression of Junction Protein and Proliferation in Mouse Sertoli Cells

  • 摘要: 目的 探讨苯并a芘(BaP)在不引起小鼠睾丸支持细胞凋亡的情况下,对缝隙连接蛋白43(CX43)表达及细胞增殖的作用。

    方法 取小鼠睾丸支持细胞系——TM4细胞,以二甲基亚砜为对照,0.5、10.0μmol/L浓度的BaP同时染毒培养的TM4细胞,并于染毒的4 h、72 h后收集细胞,检测细胞的活力、增殖、凋亡情况,以及CX43的mRNA和蛋白的表达量。

    结果 与对照组相比:BaP染毒4 h时,两个染毒组细胞存活率、增殖、凋亡和CX43蛋白表达没有明显变化(P > 0.05),而CX43 mRNA的表达升高(P < 0.05);72 h时,两个染毒组细胞凋亡无明显变化,而细胞存活率和增殖均下降(P < 0.05),CX43 mRNA和蛋白表达出现上升(P < 0.05)。

    结论 0.5、10.0μmol/L浓度的BaP染毒小鼠睾丸支持细胞,可抑制细胞增殖,引起CX43 mRNA和蛋白表达升高。

     

    Abstract: Objective To investigate the effects of benzoapyrene (BaP) on the expression of connexin 43(CX43) and cell proliferation in mouse sertoli cells without change of cell apoptosis.

    Methods The mouse sertoli cell line TM4 cells were exposed to varied concentrations of BaP (dimethyl sulfoxide control group,0.5 and 10.0 μmol/L BaP groups) for 4 h or 72 h.Then these cells were collected to measure cell viability,proliferation,and apoptosis.Besides,mRNA and protein expression levels of CX43 were also measured.

    Results Compared with the control group,no difference in the viability,proliferation,apoptosis,or protein expression levels of CX43 was found in the TM4 cells with 4 h-exposure to 0.5 and 10.0 μmol/L BaP (P > 0.05),but the mRNA expression levels of CX43 was significantly increased (P < 0.05).For 72 h exposure,the viability and proliferation were significantly decreased (P < 0.05) in the TM4 cells without change of apoptosis,and the mRNA and protein expression levels of CX43 were significantly increased (P < 0.05).

    Conclusion BaP exposure at 0.5 and 10.0 μmol/L could inhibit proliferation of mouse sertoli cells and increase mRNA and protein expression levels of CX43.

     

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