彭海兵, 储金秀, 曹福源, 陈松, 安春娜, 秦丽娟, 韩淑英. 熊果酸对矽肺大鼠的早期抗氧化作用[J]. 环境与职业医学, 2015, 32(5): 476-480. DOI: 10.13213/j.cnki.jeom.2015.14599
引用本文: 彭海兵, 储金秀, 曹福源, 陈松, 安春娜, 秦丽娟, 韩淑英. 熊果酸对矽肺大鼠的早期抗氧化作用[J]. 环境与职业医学, 2015, 32(5): 476-480. DOI: 10.13213/j.cnki.jeom.2015.14599
PENG Hai-binga , CHU Jin-xiub , CAO Fuyuanc , CHEN Songc , AN Chun-nab , QIN Li-juanb , HAN Shu-yingb . Antioxidation Effect of Ursolic Acid on Early Silicosis in Rats[J]. Journal of Environmental and Occupational Medicine, 2015, 32(5): 476-480. DOI: 10.13213/j.cnki.jeom.2015.14599
Citation: PENG Hai-binga , CHU Jin-xiub , CAO Fuyuanc , CHEN Songc , AN Chun-nab , QIN Li-juanb , HAN Shu-yingb . Antioxidation Effect of Ursolic Acid on Early Silicosis in Rats[J]. Journal of Environmental and Occupational Medicine, 2015, 32(5): 476-480. DOI: 10.13213/j.cnki.jeom.2015.14599

熊果酸对矽肺大鼠的早期抗氧化作用

Antioxidation Effect of Ursolic Acid on Early Silicosis in Rats

  • 摘要: 目的 观察熊果酸(ursolic acid)对矽肺大鼠早期抗氧化作用。

    方法 将60只SPF级Wistar雄性大鼠,随机分为对照组、模型组及熊果酸低、中、高剂量组、溶剂对照组,每组10只。除对照组外,其余各组大鼠采用非暴露法气管内一次性注入二氧化硅(SiO2)悬液(250 mg/kg)复制动物矽肺模型。熊果酸低、中、高剂量组自注射SiO2后每天灌胃熊果酸10、20、40 mg/kg,溶剂对照组每天灌胃0.6%羧甲基纤维素钠溶液(10 mL/kg),对照组灌胃生理盐水(10 mL/kg),连续56 d。检测28 d各组大鼠肺脏器系数变化,检测大鼠血清一氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量,及肺组织核转录因子κB (NF-κB)蛋白表达情况。

    结果 模型组和溶剂对照组大鼠肺脏器系数、血清NO、MDA含量、NF-κB蛋白表达高于对照组,而SOD、GSH-Px含量低于对照组,差异均有统计学意义(P < 0.05)。与模型组和溶剂对照组比较,熊果酸各剂量组大鼠肺脏器系数、血清NO、MDA含量、NF-κB蛋白表达降低;SOD、GSH-Px含量升高,差异均有统计学意义(P < 0.05),以上变化均与熊果酸呈现剂量依赖效应。

    结论 熊果酸通过纠正氧化抗氧化系统失衡,抑制NF-κB的活化而减缓矽肺纤维化的发生。

     

    Abstract: Objective To explore the antioxidation effect of ursolic acid on early silicosis in rats by experiment study.

    Methods Totally 60 SPF Wistar rats were randomly divided into six groups:control (NS),model (M),and,low (UA10),medium (UA20),and high ursolic acid (UA40),and solvent control groups (S)(n=10/group).Except the group NS,the rats in other groups were induced by intratracheal instillation of silica in suspension (250 mg/kg) to establish silicosis model.Ursolic acid at 10,20,and 40 mg/kg was then injected by gavage into the rats in the group UA10,UA20,and UA40 respectively.The group S were gavaged daily with 0.6% sodium carboxymethyl cellulose (10 mL/kg) solution,while the group NS was given sodium chloride (10 mL/kg) for 56 consecutive days.Pulmonary organ coefficient on 28 d was calculated for each group.Serum nitric oxide (NO),malondialdehyde (MDA),superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) contents were also measured.The expression of nuclear factor kappa B (NF-κB) protein in lung tissue was evaluated.

    Results Compared with the group NS,the pulmonary organ coefficients,serum NO and MDA contents,and NF-κB protein expression were elevated,while the SOD and GSH-Px contents were decreased in the group M and group S (P < 0.05).However,the up-regulation of pulmonary organ coefficients,serum NO and MDA contents,and NF-κB protein expression and the down-regulation of SOD and GSH-Px observed in the group M and group S were significantly reversed by the ursolic acid treatment and showed a dose-dependent effect (P < 0.05).

    Conclusion Ursolic acid could slow silicosis fibrosis by correcting the imbalance of antioxidant system and inhibiting NF-κB activation.

     

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