刘文佳, 宋金燕, 杨维超, 晋乐飞, 王威, 姚武, 吴卫东, 吴逸明, 燕贞. 人单核细胞对煤焦沥青烟提取物诱导人永生化支气管上皮细胞c-Jun mRNA表达的影响[J]. 环境与职业医学, 2015, 32(8): 786-789. DOI: 10.13213/j.cnki.jeom.2015.14516
引用本文: 刘文佳, 宋金燕, 杨维超, 晋乐飞, 王威, 姚武, 吴卫东, 吴逸明, 燕贞. 人单核细胞对煤焦沥青烟提取物诱导人永生化支气管上皮细胞c-Jun mRNA表达的影响[J]. 环境与职业医学, 2015, 32(8): 786-789. DOI: 10.13213/j.cnki.jeom.2015.14516
LIU Wen-jia , SONG Jin-yan , YANG Wei-chao , JIN Yuefei , WANG Wei , YAO Wu , WU Wei-dong , WU Yi-ming , YAN Zhen . Effect of Human Acute Monocytic Leukemia Cells on Coal Tar Pitch Extract-Induced c-Jun mRNA Expression in Human Bronchial Epithelial Cells[J]. Journal of Environmental and Occupational Medicine, 2015, 32(8): 786-789. DOI: 10.13213/j.cnki.jeom.2015.14516
Citation: LIU Wen-jia , SONG Jin-yan , YANG Wei-chao , JIN Yuefei , WANG Wei , YAO Wu , WU Wei-dong , WU Yi-ming , YAN Zhen . Effect of Human Acute Monocytic Leukemia Cells on Coal Tar Pitch Extract-Induced c-Jun mRNA Expression in Human Bronchial Epithelial Cells[J]. Journal of Environmental and Occupational Medicine, 2015, 32(8): 786-789. DOI: 10.13213/j.cnki.jeom.2015.14516

人单核细胞对煤焦沥青烟提取物诱导人永生化支气管上皮细胞c-Jun mRNA表达的影响

Effect of Human Acute Monocytic Leukemia Cells on Coal Tar Pitch Extract-Induced c-Jun mRNA Expression in Human Bronchial Epithelial Cells

  • 摘要: 目的 探讨人单核细胞对煤焦沥青烟提取物(CTPE)处理人永生化支气管上皮细胞中c-Jun mRNA表达的影响。

    方法 用3 μg/mL CTPE刺激人永生化支气管上皮细胞(CTPE组),及人单核细胞共培养的人永生化支气管上皮细胞(CC组),设立二甲基亚砜对照组;分别收集各组1、5、10、15、20代细胞。将CC组第9代细胞分别常规培养至10代(CC10组)、15代(CC15组),加入100 μg/mL肿瘤坏死因子-α中和抗体培养至15代(中和抗体组)。应用实时荧光定量聚合酶链式反应检测各组细胞中c-Jun mRNA的表达水平。

    结果 15、20代CC组人永生化支气管上皮细胞c-Jun mRNA相对表达水平高于CTPE组和二甲基亚砜对照组(均P<0.05);中和抗体组c-Jun mRNA的相对表达水平高于CC10组,低于CC15组(均P<0.05)。

    结论 人单核细胞可能通过肿瘤坏死因子-α调控CTPE诱导人永生化支气管上皮细胞c-Jun mRNA的表达。

     

    Abstract: Objective To explore the effect of human acute monocytic leukemia cells (THP-1) on c-Jun mRNA expression induced by coal tar pitch extract (CPTE) in human bronchial epithelial cells (BEAS-2B).

    Methods BEAS-2B cells (CTPE group) and co-cultured BEAS-2B and THP-1 cells (CC group) were both simulated by 3μg/mL CTPE, and dimethyl sulfoxide (DMSO) was used as vehicle control. The cells were collected at passage 1, 5, 10, 15, and 20. The co-cultured cells at passage 9 were further cultured to passage 10 (CC10 group) and passage 15 (CC15 group), and added 100 μg/mL tumor necrosis factor-α (TNF-α) neutralizing antibody and further cultured to passage 15 (neutralizing antibody group). The expression of c-Jun mRNA was detected by real-time fluorescent quantitative polymerase chain reaction.

    Results The expression levels of c-Jun mRNA in BEAS-2B cells at passage 15 and 20 in the CC group were both increased compared with those in the CTPE and DMSO control group (P<0.05). The expression level of c-Jun mRNA after adding TNF-α neutralizing antibody was increased compared with the CC10 group and decreased compared with the CC15 group (P<0.05).

    Conclusion THP-1 could regulate the expression of c-Jun mRNA induced by CTPE through TNF-α in the BEAS-2B cells.

     

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