杨飞玉, 王丽, 高艳荣, 耿敏杰, 米亚柯, 张永明, 隋洋洋, 李雪. 应用微核实验和彗星电泳实验评价氯化镧90 d喂养对小鼠遗传毒性作用[J]. 环境与职业医学, 2015, 32(4): 351-354. DOI: 10.13213/j.cnki.jeom.2015.14454
引用本文: 杨飞玉, 王丽, 高艳荣, 耿敏杰, 米亚柯, 张永明, 隋洋洋, 李雪. 应用微核实验和彗星电泳实验评价氯化镧90 d喂养对小鼠遗传毒性作用[J]. 环境与职业医学, 2015, 32(4): 351-354. DOI: 10.13213/j.cnki.jeom.2015.14454
YANG Fei-yu , WANG Li , GAO Yan-rong , GENG Min-jie , MI Yake , ZHANG Yong-ming , SUI Yang-yang , LI Xue . Application of Micronucleus Test and Comet Assay for Lanthanum Chloride Genotoxicity Evaluationin Mice by 90-Day Feeding Method[J]. Journal of Environmental and Occupational Medicine, 2015, 32(4): 351-354. DOI: 10.13213/j.cnki.jeom.2015.14454
Citation: YANG Fei-yu , WANG Li , GAO Yan-rong , GENG Min-jie , MI Yake , ZHANG Yong-ming , SUI Yang-yang , LI Xue . Application of Micronucleus Test and Comet Assay for Lanthanum Chloride Genotoxicity Evaluationin Mice by 90-Day Feeding Method[J]. Journal of Environmental and Occupational Medicine, 2015, 32(4): 351-354. DOI: 10.13213/j.cnki.jeom.2015.14454

应用微核实验和彗星电泳实验评价氯化镧90 d喂养对小鼠遗传毒性作用

Application of Micronucleus Test and Comet Assay for Lanthanum Chloride Genotoxicity Evaluationin Mice by 90-Day Feeding Method

  • 摘要: 目的 应用微核实验和彗星电泳实验评价氯化镧亚慢性染毒致小鼠遗传毒性作用。

    方法 将100只SPF级ICR小鼠随机分为5组:4个氯化镧染毒组和1个对照组,每组20只,雌雄各半。5个剂量组分别以灌胃的方式给予不同浓度的氯化镧溶液(0、10、20、50和100 mg/kg)。每周染毒6次,连续染毒13周后,取小鼠外周血进行彗星实验及镧含量的测定,取小鼠骨髓观察骨髓细胞微核率。

    结果 镧染毒剂量达50 mg/kg时,彗星细胞的尾长及尾部DNA含量与对照组相比,差异有统计学意义(P < 0.05),小鼠骨髓细胞微核率与对照组相比,差异有统计学意义(P < 0.05)。4个氯化镧染毒组血液中均有不同程度的镧的蓄积,与对照组相比,差异均有统计学意义(P < 0.05)。

    结论 稀土镧元素可以在血液中蓄积,具有一定的遗传毒性。

     

    Abstract: Objective To evaluate the genotoxicity of sub-chronic exposure to lanthanum chloride on mice using micronucleus test and comet assay.

    Methods SPF grade ICR mice (n=100) were randomly divided into five groups,includingfour lanthanum chloride exposure groups and a control group,20 mice in each group,male and female half and half.The five dose groups were fed by gavage respectively with different concentrations of lanthanum chloride solution (0,10,20,50,and 100 mg/kg),6 times a week,for 13 consecutive weeks.Peripheral blood samples of mice were collected for comet assay to determine lanthanum content,and bone marrow samples were taken to observe bone marrow micronucleus rate.

    Results The comet tail length of the cells and the tail DNA content in the groups exposed to lanthanum 50 mg/kg and up were significantly different compared with the control group (P < 0.05),so were mouse bone marrow micronucleus rates (P < 0.05).The four lanthanum chloride treated groups showed varied degrees of lanthanum accumulation in blood,and showed significant differences compared with the control group (P < 0.05).

    Conclusion Lanthanum could accumulate in blood and lead to genetic toxicitygroup

     

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