宋波, 武柏林, 刘士鹏, 高天阳, 张荣, 牛玉杰. 母鼠孕前低水平铅暴露对子代骨骼钙代谢的影响[J]. 环境与职业医学, 2015, 32(2): 151-154. DOI: 10.13213/j.cnki.jeom.2015.14347
引用本文: 宋波, 武柏林, 刘士鹏, 高天阳, 张荣, 牛玉杰. 母鼠孕前低水平铅暴露对子代骨骼钙代谢的影响[J]. 环境与职业医学, 2015, 32(2): 151-154. DOI: 10.13213/j.cnki.jeom.2015.14347
SONG Bo , WU Bai-lin , LIU Shi-peng , GAO Tian-yang , ZHANG Rong , NIU Yujie . Effects of Maternal Preconceptional Low-Level Lead Exposure on Skeletal Calcium Metabolism in Newborn Mice[J]. Journal of Environmental and Occupational Medicine, 2015, 32(2): 151-154. DOI: 10.13213/j.cnki.jeom.2015.14347
Citation: SONG Bo , WU Bai-lin , LIU Shi-peng , GAO Tian-yang , ZHANG Rong , NIU Yujie . Effects of Maternal Preconceptional Low-Level Lead Exposure on Skeletal Calcium Metabolism in Newborn Mice[J]. Journal of Environmental and Occupational Medicine, 2015, 32(2): 151-154. DOI: 10.13213/j.cnki.jeom.2015.14347

母鼠孕前低水平铅暴露对子代骨骼钙代谢的影响

Effects of Maternal Preconceptional Low-Level Lead Exposure on Skeletal Calcium Metabolism in Newborn Mice

  • 摘要: 目的 研究母鼠孕前低水平铅暴露是否对子代骨骼钙代谢有影响。

    方法 12只雌性昆明小鼠随机分为铅暴露组、对照组,每组6只。铅暴露组小鼠通过饮水暴露于醋酸铅(含铅元素10.0 mol/L)4周后怀孕。在仔鼠出生后的第21天和第60天,两组每窝随机选取3只仔鼠处死,取股骨、全血和血清,用石墨炉原子吸收法测定铅含量,用火焰原子吸收法测定钙含量,用放射免疫法测定血清总甲状旁腺激素(PTH)含量。

    结果 在出生后第21天,与对照组仔鼠相比,铅暴露组仔鼠血钙水平(1.75& #177;0.24)mmol/L、骨钙水平(10.17& #177;1.03)mmol/g均明显降低,血铅水平(0.31& #177;0.03)μmol/L、骨铅水平(57.24& #177;4.65)nmol/g及血清总PTH水平(3.40& #177;0.74)μg/L均明显升高(P<0.05)。到出生后第60天,与出生后第21天相比,铅暴露组仔鼠血钙水平(2.11& #177;0.17)mmol/L明显上升(P<0.05),且与对照组仔鼠无明显差异(P>0.05);骨钙水平(13.65& #177;2.11)mmol/g、血清总PTH水平(5.91& #177;0.85)μg/L均明显上升(P <0.05),且与对照组仔鼠有明显差异(P<0.05);骨铅水平(45.04& #177;7.62)nmol/g明显下降(P<0.05),但仍明显高于对照组仔鼠(P<0.05);血铅水平(0.29& #177;0.02)μmol/L无明显改变,仍明显高于对照组仔鼠(P<0.05)。

    结论 母鼠孕前低水平铅暴露影响导致了子代小鼠骨骼钙代谢。

     

    Abstract: Objective To study whether maternal pre-pregnant low-level lead exposure could affect skeletal calcium metabolism in newborn mice.

    Methods A group of six female Kunming mice were exposed to lead acetate in drinking water (10.0 mol/L, pure lead) for four weeks before pregnancy. Another six female Kunming mice were set as controls. At the postnatal day 21 and 60, three newborn mice from each group were neutralized to detect the levels of lead, calcium, and parathyroid hormone (PTH) in samples of femur, blood, and serum by graphite furnace atomic absorption spectrometry, flame atomic absorption spectrometry, and radioimmunoassay, respectively.

    Results On postnatal day 21, compared with the control offspring, the levels of blood calcium and femur calcium were significantly lower(1.75±0.24) mmol/L and (10.17±1.03) mmol/g, respectively, P<0.05, the le vels of blood lead, femur lead, and serum PTH were significantly elevated in the lead-treated newborn mice(0.31±0.03) μmol/L, (57.24±4.65) nmol/g, and (3.40±0.74) μg/L respectively, P<0.05. On postnatal day 60, compared with the postnatal day 21 results, the levels of blood calcium in the preconceptional-lead-exposed newborn mice were increased to (2.11±0.17) mmol/L, and there were no significant differences when compared with the control offspring (P > 0.05); the levels of femur calcium and PTH were in creased to (13.65±2.11) mmol/g and (5.91±0.85) μg/L, respectively, and there were significant differences compared with the corresponding indicators of the controls (P<0.05); the levels of femur lead were decreased to (45.04±7.62) nmol/g, and significantly higher than those of control offspring (P<0.05); the levels of blood lead (0.29±0.02)μmol/L were significantly higher than those of the control offspring (P<0.05) though little change was found versus the postnatal day 21 results.

    Conclusion Maternal preconceptional low-level lead exposure could affect skeletal calcium metabolism in newborn mice.

     

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