覃利梅, 李燕, 李荣娟, 苏旭. 原子荧光光谱法测定尿锑浓度[J]. 环境与职业医学, 2015, 32(4): 363-365. DOI: 10.13213/j.cnki.jeom.2015.14319
引用本文: 覃利梅, 李燕, 李荣娟, 苏旭. 原子荧光光谱法测定尿锑浓度[J]. 环境与职业医学, 2015, 32(4): 363-365. DOI: 10.13213/j.cnki.jeom.2015.14319
QIN Li-mei , LIYan , LI Rong-juan , SU Xu . Determination of Antimony in Urine by Atomic Fluorescence Spectrometric Method[J]. Journal of Environmental and Occupational Medicine, 2015, 32(4): 363-365. DOI: 10.13213/j.cnki.jeom.2015.14319
Citation: QIN Li-mei , LIYan , LI Rong-juan , SU Xu . Determination of Antimony in Urine by Atomic Fluorescence Spectrometric Method[J]. Journal of Environmental and Occupational Medicine, 2015, 32(4): 363-365. DOI: 10.13213/j.cnki.jeom.2015.14319

原子荧光光谱法测定尿锑浓度

Determination of Antimony in Urine by Atomic Fluorescence Spectrometric Method

  • 摘要: 目的 建立原子荧光光谱法测定尿锑浓度的检测方法。

    方法 尿样经混合酸消化后,加入硫脲和抗坏血酸使5价锑还原为3价锑,再加入硼氢化钾还原生成锑化氢,由氩气载入石英原子化器中分解为原子态锑,在锑空心阴极灯发射光的激发下产生原子荧光,荧光强度与标准系列比较定量。

    结果 尿锑浓度为0.0~10.0 μg/L的线性关系较好,相关系数为0.999 4~0.999 9;最低检出限为0.06 μg/L;相对标准偏差为1.9%~2.4%;平均加标回收率为99.7%。

    结论 建立的湿法消解-氢化物发生-原子荧光光谱法测定尿锑实用、快速、准确、灵敏度高、基体干扰少,便于推广运用,尤其适用于大批量人群职业健康体检尿锑的检测。

     

    Abstract: Objective To develoP an atomic fluorescence spectrometric method for detecting the concentration of antimony in urine.

    Methods Urine samples were digested using mixed acids and treated with thiourea and ascorbic acid to reduce pentavalent antimony to trivalent antimony.Then potassium borohydride was added to generate stibine which was afterwards decomposed to atomic antimony in quartz furnace atomizer using argon gas.Fluorescence was excited by an incident light from a hollow cathode lamp,and fluorescence intensity was compared with relevant standards to quantify antimony.

    Results A good linear relationship for antimony was found in the range of 0.0-10.0 μg/L,and the correlation coefficients were 0.999 4-0.999 9.The lowest limit of detection was 0.06 μg/L;the relative standard deviations were 1.9%-2.4%;the average recovery was 99.7%.

    Conclusion The wet digestion hydride generation atomic fluorescence spectrometric method for determination of urine antimony is practical,fast,accurate,highly sensitive,less interfered and easy to promote.Therefore,it is recommended for mass detection of urine antimony in occupational health surveillancegroup

     

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