吴立梦, 林庆能, 王文静, 许慧慧, 张曦. 环境水体中感染性胃肠道病毒检测方法研究进展[J]. 环境与职业医学, 2014, 31(6): 490-493,497. DOI: 10.13213/j.cnki.jeom.2014.0118
引用本文: 吴立梦, 林庆能, 王文静, 许慧慧, 张曦. 环境水体中感染性胃肠道病毒检测方法研究进展[J]. 环境与职业医学, 2014, 31(6): 490-493,497. DOI: 10.13213/j.cnki.jeom.2014.0118
WU Li-meng , LIN Qing-neng , WANG Wen-jing , XU Hui-hui , ZHANG Xi . Research Progress on Detection of Infectious Enteric Viruses in Environmental Water[J]. Journal of Environmental and Occupational Medicine, 2014, 31(6): 490-493,497. DOI: 10.13213/j.cnki.jeom.2014.0118
Citation: WU Li-meng , LIN Qing-neng , WANG Wen-jing , XU Hui-hui , ZHANG Xi . Research Progress on Detection of Infectious Enteric Viruses in Environmental Water[J]. Journal of Environmental and Occupational Medicine, 2014, 31(6): 490-493,497. DOI: 10.13213/j.cnki.jeom.2014.0118

环境水体中感染性胃肠道病毒检测方法研究进展

Research Progress on Detection of Infectious Enteric Viruses in Environmental Water

  • 摘要: 胃肠道病毒,是全球范围内引起经水传播的疾病的主要病原物。寻找检测水环境中具有感染性病毒的方法是当务之急。传统细胞培养是鉴定病毒感染力的金标准,但是仍存在费时、费力、成本高且有些病毒(诺如病毒)无法培养的缺点。常用的分子生物学检测方法在检测水中病毒时,虽然有很高的灵敏度和特异性,但亦存在病毒基因拷贝数和感染力之间缺乏相关性的局限性。本文遂对目前国内外在检测感染性病毒的相关研究方面,能部分克服传统细胞培养和直接聚合酶链式反应(PCR)法检测感染性病毒时之局限性的研究成果作一综述报道。

     

    Abstract: Enteric viruses are one of the leading causes of waterborne disease worldwide. It is urgent to develop rapid and sensitive methods to detect infectious viruses in water environment. Despite being a gold standard for virus infectivity detection, cell culture infectivity assays are laborious, time consuming, and costly, and some viruses such as human noroviruses have not been able to be cultured in vitro. Common molecular biological methods show high sensitivity and specificity in viruses detection in water; however, the lack of correlation between detected viral gene copy number and viral infectivity is a main limitation. This review presented a summary and critical evaluation of different approaches proposed to overcome limitations of the traditional cell culture assay and polymerase chain reaction assay.

     

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