周妍, 赵百慧, 郭家胤, 黄峥, 张曦. 上海2010-2013年H3N2流感病毒流行特征[J]. 环境与职业医学, 2014, 31(6): 426-429. DOI: 10.13213/j.cnki.jeom.2014.0098
引用本文: 周妍, 赵百慧, 郭家胤, 黄峥, 张曦. 上海2010-2013年H3N2流感病毒流行特征[J]. 环境与职业医学, 2014, 31(6): 426-429. DOI: 10.13213/j.cnki.jeom.2014.0098
ZHOU Yan , ZHAO Bai-hui , GUO Jia-yin , HUANG Zheng , ZHANG Xi . Epidemiologic Characteristics of H3N2 Isolates in Shanghai,2010-2013[J]. Journal of Environmental and Occupational Medicine, 2014, 31(6): 426-429. DOI: 10.13213/j.cnki.jeom.2014.0098
Citation: ZHOU Yan , ZHAO Bai-hui , GUO Jia-yin , HUANG Zheng , ZHANG Xi . Epidemiologic Characteristics of H3N2 Isolates in Shanghai,2010-2013[J]. Journal of Environmental and Occupational Medicine, 2014, 31(6): 426-429. DOI: 10.13213/j.cnki.jeom.2014.0098

上海2010-2013年H3N2流感病毒流行特征

Epidemiologic Characteristics of H3N2 Isolates in Shanghai,2010-2013

  • 摘要: 目的 了解上海市2010-2013年流行的甲3流感病毒血凝毒(HA)基因突变及其抗原变异情况与流感流行的关系。

    方法 用实时荧光聚合酶链反应(real-time PCR)检测2010-2013年因类流感症状(ILI)到流感哨点医院就诊的监测病例,分析甲3亚型流感病毒流行特征。鸡胚传代甲3亚型阳性标本,收获尿囊腔液用作提取病毒的RNA,进行逆转录聚合酶链扩增,扩增产物纯化后进行测序,用MegAlign软件对血凝素(HA)1区域氨基酸位点进行对比分析,用MEGA软件对HA基因进化树进行分析。

    结果 2010-2013年间,上海市甲3亚型流感病毒为2010、2012、2013年主要流行株。流行特征具有明显的年度和季节特点,与全国一致。与2011年世界卫生组织推荐的疫苗株相比,上海市甲3亚型流感病毒HA1区域在2010年发生明显抗原改变,共发生35处氨基酸替代,其中23处位点改变较大, 6个氨基酸位点涉及HA1的4个抗原决定簇。2011年后的毒株在进化树上也形成一个独立的分支。

    结论 上海市2012年较大规模的甲3亚型流感病毒流行与病毒的抗原性漂移有关。

     

    Abstract: Objective To examine the relationship of influenza A virus HA genetic drift and its antigenic drift with the epidemiologic situation of H3N2 circulated in Shanghai from 2010 to 2013.

    Methods Real-time polymerase chain reactor was used to test the patients with influenza like illness (ILI) who visited Shanghai influenza sentinel hospitals and analyze epidemiologic characteristics of H3N2 viruses. Embryonated eggs were inoculated with virus for amplification of viral yield, and allantoic fluids from virus positive embryos were used to extract viral RNA. HA gene sequences were amplified by reverse transcription polymerase chain reaction and sequenced with ABI 3730xl. The amino acid changes were analyzed with MegAlign software and the phylogenic tree was drawn by MEGA software.

    Results H3N2 subtype was the dominant influenza strain in the years of 2010, 2012, and 2013. A seasonal variation of H3N2 influenza incidence in Shanghai was recognized, which concurred with the other regions of China. The H3N2 viruses isolated in 2010 were different in amino acid sequences on HA1 domain protein molecule from the WHO vaccine strains in 2011. There were 35 amino acid substitutions, among which twenty-three positions showed significant changes and six lie in four antigen determinant regions. The phylogenic tree showed the strains isolated after 2011 belonged to a different branch.

    Conclusion Antigenic drift of influenza subtype H3N2 is correlated with the influenza epidemics in Shanghai.

     

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