陶功华, 王莉萍, 俞筱琦, 施志冲, 洪新宇, 帅怡, 王宁, 肖萍. α-硫辛酸对2型糖尿病大鼠氧化应激致胰岛细胞凋亡的影响[J]. 环境与职业医学, 2014, 31(4): 247-251,271. DOI: 10.13213/j.cnki.jeom.2014.0058
引用本文: 陶功华, 王莉萍, 俞筱琦, 施志冲, 洪新宇, 帅怡, 王宁, 肖萍. α-硫辛酸对2型糖尿病大鼠氧化应激致胰岛细胞凋亡的影响[J]. 环境与职业医学, 2014, 31(4): 247-251,271. DOI: 10.13213/j.cnki.jeom.2014.0058
TAO Gong-hua , WANG Li-ping , YU Xiao-qi , SHI Zhi-chong , HONG Xin-yu , SHUAI Yi , WANG Ning , XIAO Ping . Effects of Alpha-Lipoic Acid on Oxidative Stress Induced Pancreatic Islet Cell Apoptosis in Type 2 Diabetic Rats[J]. Journal of Environmental and Occupational Medicine, 2014, 31(4): 247-251,271. DOI: 10.13213/j.cnki.jeom.2014.0058
Citation: TAO Gong-hua , WANG Li-ping , YU Xiao-qi , SHI Zhi-chong , HONG Xin-yu , SHUAI Yi , WANG Ning , XIAO Ping . Effects of Alpha-Lipoic Acid on Oxidative Stress Induced Pancreatic Islet Cell Apoptosis in Type 2 Diabetic Rats[J]. Journal of Environmental and Occupational Medicine, 2014, 31(4): 247-251,271. DOI: 10.13213/j.cnki.jeom.2014.0058

α-硫辛酸对2型糖尿病大鼠氧化应激致胰岛细胞凋亡的影响

Effects of Alpha-Lipoic Acid on Oxidative Stress Induced Pancreatic Islet Cell Apoptosis in Type 2 Diabetic Rats

  • 摘要: 目的 研究α-硫辛酸对2型糖尿病大鼠氧化应激致胰岛细胞凋亡的影响。

    方法 采用高热量饮食联合腹腔注射低剂量链脲菌素制备2型糖尿病大鼠模型(模型对照组),予以α-硫辛酸(15、30、60和120 mg/kg 体质量)干预处理12周后,检测大鼠胰腺组织氧化应激指标和胰岛细胞凋亡情况,同时检测胰腺组织中凋亡相关蛋白的表达改变。

    结果 与模型组相比,各干预组大鼠胰腺组织中丙二醛含量均明显降低(P<0.01),总超氧化物歧化酶和谷胱甘肽过氧化物酶活性则明显升高(P<0.01);除15 mg/kg 体质量组外,其余各干预组大鼠胰岛凋亡指数较模型组均明显降低(P<0.01),其中以60 mg/kg 体质量干预组效果最好。与模型组比较,各干预组大鼠胰腺组织中Bax、Fas、活化型Caspase-9和Caspase-3等蛋白表达均降低,而Bcl-2蛋白表达则明显增高。

    结论 α-硫辛酸可通过调节大鼠胰腺组织的氧化应激状态,改变凋亡相关蛋白的表达水平,显著降低2型糖尿病模型大鼠胰岛细胞的凋亡率,增加机体胰岛素的分泌水平。

     

    Abstract: Objective To assess the effects of alpha-lipoic acid on oxidative stress induced pancreatic islet cell apoptosis in type 2 diabetic rats.

    Methods High-calorie diet combined with intraperitoneal injection of low-dose streptozotocin was used to establish a type 2 diabetic rat model. Then the model rats were treated by alpha-lipoic acid (15, 30, 60, and 120 mg/kg body weight) for 12 weeks before detecting pancreatic oxidative stress, apoptosis of pancreatic cells, and changes in the expression levels of pancreatic apoptosis-related proteins.

    Results The pancreatic malondialdehyde levels were significantly decreased in all alpha-lipoic acid intervented groups (P<0.01), while the total superoxide disumutase and the glutathione peroxidase activities were significantly increased (P<0.01), compared with the model control rats. The apoptotic indices were significantly decreased (P<0.01) in all intervention groups except the 15 mg/kg body weight group, compared with the model control rats, and the highest intervention effect was found in the 60 mg/kg body weight group. The protein levels of Bax, Fas, activated Caspase-9 and Caspase-3 decreased, and the Bcl-2 protein level increased significantly in all intervention groups.

    Conclusion Apoptosis rates of pancreatic cells are down-regulated by alpha-lipoic acid via ameliorating pancreatic oxidative stress and related protein levels.

     

/

返回文章
返回