Background Diisodecyl phthalate (DiDP) is a new type of plasticizer, and can enter the human body through a variety of ways. At present, there are few domestic studies on the nephrotoxicity of DiDP, and whether DiDP can cause renal injury in laboratory animals through oxidative stress is still unclear.
Objective This experiment investigates the effects of different doses of DiDP on the kidney in male Balb/c mice, and explores the underlying mechanism.
Methods Balb/c mice were randomly divided into control group (saline), DiDP groups (0.15, 1.5, 15, and 150 mg/kg), vitamin E (Vit E) group (100 mg/kg), and Vit E intervention group (150 mg/kg DiDP+100 mg/kg Vit E) (n=8 in each group). After 14 d of the designed exposure by gavage, the pathological changes of renal tissue specimens were observed; reactive oxygen species (ROS), malondialdehyde (MDA), reduced glutathione (GSH), and 8-hydroxydeoxyguanosine (8-OHdG) in renal tissues, as well as urea nitrogen (UREA) and creatinine (CREA) in serum were measured.
Results The levels of ROS, MDA, and 8-OHdG in renal tissues and the levels of UREA and CREA in serum were significantly higher in the 150 mg/kg DiDP group than those in the control group, and the level of GSH was significantly lower (P < 0.05). Moreover, the pathological results showed that higher doses of DiDP resulted in more serious renal injury. Compared with the 150mg/kg DiDP group, the levels of ROS, MDA, and 8-OHdG in renal tissues and the levels of UREA and CREA in serum were decreased, and the GSH level was increased in the Vit E intervention group (P < 0.05). Meanwhile, the pathological results showed that Vit E somewhat relieved the renal injury caused by 150 mg/kg DiDP.
Conclusion DiDP can induce pathological damage of renal tissues in mice through oxidative stress.
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