Objective To investigate the effects of organic extracts of source water from the Baotou section of the Yellow River on the proliferation and oxidative damage of primary germ cells of male rats.
Methods Organic matters extracted from samples of the upstream and downstream of Baotou section of the Yellow River and of tap water were administered to primary germ cells in vitro of male rats at different concentrations (10, 100, and 1 000 mL/mL; 1 mL organic extracts were equivalent to 10, 100, and 1000mL water samples, respectively) for 24h.The control group was given PBS at the same volume.Proliferation rate of cells was detected by MTT assay, apoptosis by transmission electron microscopy (TEM), and lactate dehydrogenase (LDH), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and malondialdehyde (MDA) by micro plate method.
Results Compared with the control group, the proliferation rates of germ cells exposed to 100 and 1 000 mL/mL organic extracts from tap water, upstream of the Yellow River, and downstream of the Yellow River were significantly decreased (Ps < 0.05), and the proliferation rates of the 1 000 mL/mL groups were reduced to (85.08±0.70)%, (90.80±0.30)%, and (87.50±2.35)%, respectively.Under the transmission electron microscope, the 1 000 mL/mL groups showed cell chromatin clustered at the edge of nucleus, nuclear fragmentation, scattering of some chromatin blocks in membrane, broken mitochondrial structure, vacuolated cells, and cell apoptosis.The LDH activities of the 1 000 and 100 mL/mL downstream groups and the tap water groups were significantly increased compared with that of the control group (Ps < 0.05); the activities of LDH of the 1 000 mL/mL tap water, upstream, and downstream groups were (2 417.48±298.50), (1 870.37±157.95), and (2 072.90±195.11) U/L, respectively.The activities of T-AOC and SOD of the 1 000 and 100 mL/mL tap water, upstream, and downstream groups were significantly lower than those of the control group, and the level of MDA was higher (Ps < 0.05); the activity of SOD of the 1000mL/mL tap water group(6.52±1.77)U/mL was significantly lower than that of the 1 000 mL/mL upstream group(8.74±0.90) U/mL (P < 0.05), and the level of MDA was higher(3.14±1.18) nmol/L vs.(2.27±0.82) nmol/L (P < 0.05).
Conclusion The organic matters extracted from the upstream and downstream of the Baotou section of the Yellow River could inhibit proliferation and promote apoptosis of primary germ cells of male rats.Oxidative damage may be one of the potential mechanisms.
DownLoad: