GUAN Jie-qiong, ZHOU Yuan, CHEN Wei-yue, WU Hong-mei, JU Jing-juan. Behavioral toxicity of triclosan on parent and progeny generations of Caenorhabditis elegans[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 546-552. DOI: 10.13213/j.cnki.jeom.2018.18122
Citation: GUAN Jie-qiong, ZHOU Yuan, CHEN Wei-yue, WU Hong-mei, JU Jing-juan. Behavioral toxicity of triclosan on parent and progeny generations of Caenorhabditis elegans[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 546-552. DOI: 10.13213/j.cnki.jeom.2018.18122

Behavioral toxicity of triclosan on parent and progeny generations of Caenorhabditis elegans

  • Objective To evaluate and compare the behavioral toxicity of triclosan (TCS) at different concentrations in parent and progeny of Caenorhabditis elegans (C.elegans).

    Methods One solvent control group (T0), two environmental concentration groupsT1 (2.9×10-2 μg/L TCS)and T2 (2.9 μg/L TCS), and two high concentration groupsT3 (2.9×102μg/L TCS)and T4 (2.9×104μg/L TCS) were established.After exposing agesynchronized L3 larvae of C.elegans (P0) to designed TCS levels for 72 h, the progeny nematodes were harvested and divided into three subgroups for another 48 h culture.Group A progeny (AF1) was treated without TCS; group B progeny (BF1) was continuously exposed to TCS consistent with the parental exposure concentration; group C progeny (CF1) was exposed to TCS inconsistent with the parental exposure concentration, including the following pairs:non-TCS parental exposure & low TCS progeny exposure (T0T1), low parental exposure & high progeny exposure (T1T4), and high parental exposure & low progeny exposure (T4T1).The body bend frequency and head thrash frequency of P0, AF1, BF1, and CF1 were measured as behavioral toxicity indicators.

    Results Compared to the controls, the head thrash frequency of P0 decreased only at 2.9×104μg/L TCS (P < 0.05), and no significant change was shown in the body bend frequency and the head thrash frequency of AF1 groups (P > 0.05); the body bend frequency of BF1 increased by 10.4% at 2.9×10-2 μg/L TCS (P < 0.05) and then decreased by 15.4% and 30.1% at 2.9×102 and 2.9×104 μg/L TCS, respectively (P < 0.05); the head thrash frequency of BF1 decreased with higher TCS concentrations, and showed significant differences at 2.9 μg/L and above (Ps < 0.05).The head thrash frequency of BF1 decreased at 2.9×10-2 and 2.9 μg/L TCS compared with P0 and AF1 (P < 0.05).Both the head thrash frequency and the body bend frequency were inhibited when the CF1 group was exposed to the T4T1 mode (P < 0.05); only the body bend frequency was inhibited in the CF1 group exposed to the T1T4 mode (P < 0.05).

    Conclusion The behavioral toxicity of TCS on parent and progeny generations of C.elegans varies with exposure concentrations and exposure modes, indicating that environmental exposure to TCS may lead to potential adverse health effects.

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