LI Ming-yan , TONG Jun-wang , ZENG Yang , CAO Fu-yuan , ZHEN Yongzhan , LIU Nan , YAN Li-cheng , XUE Ling , JIANG Shou-fang . Growth and Neurobehavior of Offspring Following Maternal Exposure to Fluoride and Arsenic during Gestation and Lactation Periods[J]. Journal of Environmental and Occupational Medicine, 2014, 31(1): 44-47. DOI: 10.13213/j.cnki.jeom.2014.0012
Citation: LI Ming-yan , TONG Jun-wang , ZENG Yang , CAO Fu-yuan , ZHEN Yongzhan , LIU Nan , YAN Li-cheng , XUE Ling , JIANG Shou-fang . Growth and Neurobehavior of Offspring Following Maternal Exposure to Fluoride and Arsenic during Gestation and Lactation Periods[J]. Journal of Environmental and Occupational Medicine, 2014, 31(1): 44-47. DOI: 10.13213/j.cnki.jeom.2014.0012

Growth and Neurobehavior of Offspring Following Maternal Exposure to Fluoride and Arsenic during Gestation and Lactation Periods

  • Objective To observe the effects on growth and neurobehavior of offspring rats of maternal exposure to fluoride, arsenic, and fluoride-arsenic combination during gestation and lactation periods.

    Methods Eighty SD rats (SPF) were mated according to male-to-female ratio of 1:1. Pregnant rats were randomly divided into 4 groups including control, fluoride, arsenic and fluoride-arsenic groups, each containing 10 pregnant rats. The pregnant rats in the 3 exposure groups were exposed to 100 mg/L NaF, 75 mg/L NaAsO2, and 100 mg/L NaF+75 mg/L NaAsO2 mixed solutions through drinking water ad libitum, respectively, until postnatal day 21 (PND21) of offsprings. The pregnant rats in the control group drank tap water. Changes of offspring body weight, physiological development, and neurobehavior reflect actions were observed.

    Results The body weights of the PND4 and PND21 pups in the arsenic group were (10.70& #177;1.38) and (43.72& #177;9.46)g respectively, the body weights of the PND4, PND7, PND14, and PND21 pups in the fluoride-arsenic group were (9.65& #177;1.09), (14.73& #177;1.77), (28.99& #177;2.29), and (42.64& #177;6.87)g respectively, and all of them were statistically lower than the body weights of the corresponding control pups (11.92& #177;2.46), (16.56& #177;2.41), (31.03& #177;4.16), and (48.73& #177;7.35)g(P<0.05). The pups of the 3 exposure groups showed no difference in physiological development indices including pinna detachment, eye opening, incisor growth, and hair growth in comparison with the control pups. Compared with the control pups, the positive rates of surface righting reflex and negative geotaxis reflex in the fluoride exposed PND5 pups and the positive rates of negative geotaxis reflex in the fluoride exposed PND6 pups were remarkably decreased (P<0.0083); the positive rate of negative geotaxis reflex in the arsenic exposed PND6 pups and the positive rates of negative geotaxis reflex and cliff avoidance in the fluoride-arsenic exposed PND6 pups were significantly decreased (P<0.008 3). The forelimb hanging time of the PND13 and PND14 pups in the arsenic group(6.37& #177;4.16) and (7.39& #177;4.17)s and the fluoride-arsenic group(6.00& #177;3.15) and (7.46& #177;3.07)s were obviously shorter than those of the control pups (8.82& #177;5.41) and (10.01& #177;5.09)s(P<0.05).

    Conclusion Arsenic and arsenic-fluoride joint exposure to an adult rat during gestation and lactation period may decrease body weight of offspring rats. Fluoride, arsenic, and their joint exposure during these periods may affect neurobehavioral reflect functions of offspring rats.

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