[Objective] To improve a method on determination of lead in blood by graphite furnace atomic absorption spectrometry and to estimate the uncertainty of the application.
[Methods] Mixed solution containing 0.20% nitric acid and 0.10% Triton X-100 was used as diluent, and mixed solution containing 0.10% palladium nitrate and 0.06% magnesium nitrate was used as matrix modifier. Referring to the recommended instrument measuring conditions, an AA-900T graphite furnace atomic absorption spectrophotometer was applied to determine lead in blood. The limits of detection and the lower limit of quantification of the proposed revised measurement were estimated. The accuracy were determined by national standard reference material.
[Results] The detection limit was 0.84 μg/L and the quantification lower limit was 2.80 μg/L. The linear range was 2.80-200.00 μg/L, and the relative standard deviation was 1.24% for blood lead at 100.0 μg/L. The concentration of the national standard reference material was 112.10 μg/L and within the required uncertainty range of (120.00& #177;15.00) μg/L.
[Conclusion] All parameters of the proposed method meet the requirement of the national clinic laboratory technique guideline. The estimated uncertainty of the method falls within the acceptable range.
atomic absorption spectrometry;
uncertainty of measurement