ZHAO Qian-kui , GU Xi-an , ZHOU Zhi-jun , ZHOU Yuan-ling . Study on Early Effect Biomarkers of Peripheral Nervous System Damage Induced by n-Hexane[J]. Journal of Environmental and Occupational Medicine, 2011, 28(7): 387-392.
Citation: ZHAO Qian-kui , GU Xi-an , ZHOU Zhi-jun , ZHOU Yuan-ling . Study on Early Effect Biomarkers of Peripheral Nervous System Damage Induced by n-Hexane[J]. Journal of Environmental and Occupational Medicine, 2011, 28(7): 387-392.

Study on Early Effect Biomarkers of Peripheral Nervous System Damage Induced by n-Hexane

  • Objective To determine the valuable significance of myelin basic protein (MBP), neuron-specific enolase (NSE), nerve growth factor(NGF), and neurofilament(NF) in serum as early effect biomarkers of peripheral nervous system damage induced by n-hexane.

    Methods n-Hexane was administered by gavage to rats sub-chronically. Peripheral nerve damage was verified by specific symptoms and pathological examination. Simultaneously MBP, NSE, NGF, and NF levels in serum of rats were detected.

    Results Typical symptoms of multiple peripheral neuropathy appeared in rats exposed to n-hexane, nerve damage was confirmed by pathological examination. Serum NSE and NF levels significantly increased starting from 1st or 2nd week after intoxicated in different dose groups, respectively (P < 0.05), the maximum raise were 122.94% and 193.35% respectively. Serum NGF and MBP levels changed only in very few time points. Pearson correlation coefficient between gait score and serum NSE, NF, NGF, MBP were 0.608 (P < 0.05), 0.595 (P < 0.05), -0.284 (P > 0.05), and 0.179 (P > 0.05)respectively. Bench mark dose (BMD) and bench mark dose lower confidence limit (BMDL)calculated according to NSE and NF were about 150 mg (/kg& #183; d) and 105 mg (/kg& #183; d).

    Conclusion Serum NSE, NF were the most sensitive indicators of peripheral nervous system damage, whose increase preceded the symptom manifested in the animals intoxicated and changed substantially, so they could be used as good biomarkers of toxic effect induced by n-hexane. NGF had poor sensitivity in this study. Significance of MBP remained to be verified if used as a biomarker of toxic effect induced by n-hexane.

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