[Objective] To explore the relationship between chromosomal damage induced by 1,3-butadiene(BD) and polymorphisms of metabolizing enzymes of CYP2E1 and GSTs.
[Methods] Cytokinesis-block micronucleus(CBMN) assay was used to detect chromosomal damage in peripheral lymphocytes of 166 1,3-butadiene exposed workers and 41 controls. PCRRFLP technique was applied to detect polymorphisms in CYP2E1 c1c2. Null genotypes of GSTT1 and GSTM1 were detected by PCR.
[Results] Compared the CBMN frequencies of the two groups we found that the frequency in exposed workers was higher than that in control group (P<0.01). Individuals with the CYP2E1 c1c2/c2c2 and GSTM1 positive genotype were observed to have significantly higher CBMN frequencies than individuals with more common genotypes (χ2=14.39, P<0.01 and χ2=4.23, P<0.05). Sex, age, duration of work, smoking and drinking habit had no relationship with CBMN frequencies.
[Conclusion] BD-exposed workers had higher risk of chromosomal damage compared with control. Cytokinesis-block micronucleus(CBMN) assay can be used to evaluate the early damage of butadiene-exposed workers. CYP2E1 c1c2/c2c2 and GSTM1 positive genotype showed higher average CBMN frequencies than those with more common genotypes.