ZHANG Ying, MA Yue, ZHENG Yu-hong, LIU Ran, PU Yue-pu, YIN Li-hong. Mechanism of LincRNA-p21 on inhibiting proliferation of esophageal cancer cells[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 479-484. DOI: 10.13213/j.cnki.jeom.2018.17712
Citation: ZHANG Ying, MA Yue, ZHENG Yu-hong, LIU Ran, PU Yue-pu, YIN Li-hong. Mechanism of LincRNA-p21 on inhibiting proliferation of esophageal cancer cells[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 479-484. DOI: 10.13213/j.cnki.jeom.2018.17712

Mechanism of LincRNA-p21 on inhibiting proliferation of esophageal cancer cells

  • Objective To investigate the underlying mechanisms of long intergenic non-coding RNA (LincRNA)-p21 on the inhibition of esophageal cancer cell proliferation.

    Methods The expression of LincRNA-p21 in two esophageal cancer cell lines (EC109 and EC9706), a human immortalized esophageal epithelial cell line (Het-1A), and the tissues and paired adjacent non-tumor mucosa of 64 cases of esophageal cancer were detected by real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR). After being transfected successfully with lentivirus packaging full-length LincRNA-p21 gene, the cell cycle distribution of EC109 cells was detected by flow cytometry, and the proliferation of EC109 cells by EdU staining. Meanwhile, RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of p21 and the protein expression level of cyclin D.

    Results The LincRNA-p21 expression levels were 0.18 and 0.32 folds in EC109 and EC9706 cells as that in Het-1A cells, respectively (P < 0.05), and the p21 mRNA levels were 0.42 and 0.48 folds, respectively. After transfection with lentivirus, the relative expression level of LincRNA-p21 increased by about 14 860 folds (P < 0.05) and that of p21-mRNA increased by 1.83 folds (P < 0.05). The results of flow cytometry showed that in the LincRNA-p21 transfection group, cells in G1 phase increased, cells in S phase and G2 phase decreased (P < 0.05), and the proliferation index was lower than that in the negative control group (40.4% vs. 48.2%) (P < 0.05). The results of EdU staining showed that the cell proliferation rate decreased after up-regulating LincRNA-p21. The results of Western blot showed that overexpression of LincRNA-p21 down-regulated the protein expression level of cyclin D.

    Conclusion LincRNA-p21 promotes the expression of p21 and inhibits the proliferation of human esophageal cancer EC109 cells by inhibiting the expression of cyclin D and contributing to G1/S arrest.

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