CAO Yu-min, XU Jie, FAN Xi-min, LUO Ying, LIU Jie, WU Qin, FAN Qi-yuan. Change of PARK2 gene expression and association with motor and memory functions of rats after demanganization[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 541-545. DOI: 10.13213/j.cnki.jeom.2018.17701
Citation: CAO Yu-min, XU Jie, FAN Xi-min, LUO Ying, LIU Jie, WU Qin, FAN Qi-yuan. Change of PARK2 gene expression and association with motor and memory functions of rats after demanganization[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 541-545. DOI: 10.13213/j.cnki.jeom.2018.17701

Change of PARK2 gene expression and association with motor and memory functions of rats after demanganization

  • Objective To explore the change of PARK2 gene expression in whole blood before and after cessation of manganese exposure in rats, and the association with motor and memory functions.

    Methods Sixty healthy adult SD rats were randomly divided into control group (saline), low dose group (MnCl2, 1 mg/kg), and high dose group (MnCl2, 5 mg/kg), with 20 rats in each group. The animals were injected intraperitoneally with MnCl2 at 5 mL/kg, once every other day. After continuous intraperitoneal injection for 4 weeks, 10 rats in each group were randomly selected for experiments on motor and memory functions such as rotarod performance test (RPT) and Y maze experiment. PARK2 gene expression in whole blood was detected by real-time fluorescence quantitative PCR (RT-PCR), and concentration of manganese in whole blood by graphite furnace atomic absorption spectrometry (AAS). The remaining rats in each group stopped intraperitoneal injection of manganese and received normal feeding for 5 months. They were anesthetized with ether every month to collect whole blood samples of infrarenal vein and detect changes of PARK2 expression.

    Results One month after intraperitoneal injection of MnCl2, the blood levels of manganese of the high dose group(5.31±0.23) mg/L and low dose group(3.13±0.16) mg/L were increased compared with the control group(2.10±0.15) mg/L (P < 0.05); the PARK2 gene expression levels of the high dose group (6.83±1.97) and the low dose group (13.15±3.84) were lower than that of the control group (26.05±3.66) (P < 0.05); the distance traveled in RPT of the high dose group(743.56±99.66) cm was lower than that of the control group(1 547.09±192.53) cm (P < 0.05); the level of manganese in whole blood was negatively correlated with the RPT distance traveled (r=-0.74) and PARK2 gene expression (r=-0.57) (Ps < 0.05); PARK2 gene expression was positively correlated with the distance traveled in RPT (r=0.61, P=0.02). After 5 months of cessation of manganese exposure, the blood level of manganese in the high dose group(2.54±0.20) mg/L was decreased (P < 0.05), but still higher than that in the control group(1.80±0.09) mg/L (P < 0.05); the distance traveled of the high dose group was (1 105.73±132.23) cm, which was higher than that before cessation of manganese exposure (P < 0.05).

    Conclusion PARK2 gene expression in whole blood and motor function of rats would recover after cessation of subacute manganese poisoning, and PARK2 gene may be used to determine the damage to motor function caused by manganese.

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