管洁琼, 周媛, 陈炜越, 邬红梅, 居静娟. 三氯生对秀丽隐杆线虫亲代及其子代的行为毒性[J]. 环境与职业医学, 2018, 35(6): 546-552. DOI: 10.13213/j.cnki.jeom.2018.18122
引用本文: 管洁琼, 周媛, 陈炜越, 邬红梅, 居静娟. 三氯生对秀丽隐杆线虫亲代及其子代的行为毒性[J]. 环境与职业医学, 2018, 35(6): 546-552. DOI: 10.13213/j.cnki.jeom.2018.18122
GUAN Jie-qiong, ZHOU Yuan, CHEN Wei-yue, WU Hong-mei, JU Jing-juan. Behavioral toxicity of triclosan on parent and progeny generations of Caenorhabditis elegans[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 546-552. DOI: 10.13213/j.cnki.jeom.2018.18122
Citation: GUAN Jie-qiong, ZHOU Yuan, CHEN Wei-yue, WU Hong-mei, JU Jing-juan. Behavioral toxicity of triclosan on parent and progeny generations of Caenorhabditis elegans[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 546-552. DOI: 10.13213/j.cnki.jeom.2018.18122

三氯生对秀丽隐杆线虫亲代及其子代的行为毒性

Behavioral toxicity of triclosan on parent and progeny generations of Caenorhabditis elegans

  • 摘要: 目的 观察和比较不同浓度三氯生对秀丽隐杆线虫亲代及其子代的行为毒性。

    方法 设立溶剂对照组T0, 环境浓度染毒组T1、T2(2.9×10-2、2.9μg/L);高浓度组T3、T4(2.9×102、2.9×104 μg/L)。同步化的L3期亲代(P0)线虫染毒72 h后, 同步化得到子代线虫分为3组, 继续培养48 h至成年。子代线虫包括A组子代(AF1)停止染毒;B组子代(BF1)染毒浓度与亲代一致;C组子代(CF1)染毒浓度与亲代不一致, 包括“亲无子低” “亲低子高” “亲高子低”即T0T1、T1T4、T4T1三种模式。观察P0、AF1、BF1和CF1各组线虫身体弯曲频率和头部摆动频率的行为毒性指标改变。

    结果 与对照组相比, P0仅2.9×104 μg/L组头部摆动频率下降(P < 0.05), AF1各染毒组身体弯曲频率和头部摆动频率改变均无统计学意义(P > 0.05);BF1中2.9×10-2 μg/L组身体弯曲频率升高了10.4%, 2.9×102、2.9×104 μg/L组身体弯曲频率分别下降了15.4%和30.1%(P < 0.05);BF1的头部摆动频率随染毒浓度的增加而降低, 2.9 μg/L及以上染毒组与对照组相比差异均有统计学意义(P < 0.05)。与P0、AF1相比, 2.9×10-2、2.9 μg/L三氯生染毒时, BF1头部摆动频率降低(P < 0.05)。与对照组相比, CF1中T4T1模式下线虫头部摆动频率和身体弯曲频率均降低(P < 0.05);T1T4模式下仅身体弯曲频率降低(P < 0.05)。

    结论 不同浓度三氯生对亲代及不同染毒模式对子代线虫的行为毒性不同, 提示环境中的三氯生可能具有潜在健康影响。

     

    Abstract: Objective To evaluate and compare the behavioral toxicity of triclosan (TCS) at different concentrations in parent and progeny of Caenorhabditis elegans (C.elegans).

    Methods One solvent control group (T0), two environmental concentration groupsT1 (2.9×10-2 μg/L TCS)and T2 (2.9 μg/L TCS), and two high concentration groupsT3 (2.9×102μg/L TCS)and T4 (2.9×104μg/L TCS) were established.After exposing agesynchronized L3 larvae of C.elegans (P0) to designed TCS levels for 72 h, the progeny nematodes were harvested and divided into three subgroups for another 48 h culture.Group A progeny (AF1) was treated without TCS; group B progeny (BF1) was continuously exposed to TCS consistent with the parental exposure concentration; group C progeny (CF1) was exposed to TCS inconsistent with the parental exposure concentration, including the following pairs:non-TCS parental exposure & low TCS progeny exposure (T0T1), low parental exposure & high progeny exposure (T1T4), and high parental exposure & low progeny exposure (T4T1).The body bend frequency and head thrash frequency of P0, AF1, BF1, and CF1 were measured as behavioral toxicity indicators.

    Results Compared to the controls, the head thrash frequency of P0 decreased only at 2.9×104μg/L TCS (P < 0.05), and no significant change was shown in the body bend frequency and the head thrash frequency of AF1 groups (P > 0.05); the body bend frequency of BF1 increased by 10.4% at 2.9×10-2 μg/L TCS (P < 0.05) and then decreased by 15.4% and 30.1% at 2.9×102 and 2.9×104 μg/L TCS, respectively (P < 0.05); the head thrash frequency of BF1 decreased with higher TCS concentrations, and showed significant differences at 2.9 μg/L and above (Ps < 0.05).The head thrash frequency of BF1 decreased at 2.9×10-2 and 2.9 μg/L TCS compared with P0 and AF1 (P < 0.05).Both the head thrash frequency and the body bend frequency were inhibited when the CF1 group was exposed to the T4T1 mode (P < 0.05); only the body bend frequency was inhibited in the CF1 group exposed to the T1T4 mode (P < 0.05).

    Conclusion The behavioral toxicity of TCS on parent and progeny generations of C.elegans varies with exposure concentrations and exposure modes, indicating that environmental exposure to TCS may lead to potential adverse health effects.

     

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