石墨炉原子吸收光谱法测定血铅方法的改进
Improvement on Determination of Lead in Blood by Graphite Furnace Atomic Absorption Spectrometry
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摘要:
目的 改进石墨炉检测低浓度血铅国标方法,对测定结果进行不确定度评定。 方法 应用0.20%硝酸和0.10%曲拉通X-100 混合溶液作为稀释剂,0.10%硝酸钯和0.06%硝酸镁作为基体改进剂,参考仪器推荐测定条件,应用AA-900T 石墨炉原子吸收分光光度计测定血中铅。对所建立的方法进行检出限及定量下限等方法学验证,并应用国家标准物质对准确度进行评定。 结果 结果显示:检出限为0.84 μg/L;定量下限为2.80 μg/L;线性范围为2.80~200.00 μg/L;血铅浓度为100.00 μg/L 时相对标准偏差为1.24%;对国家标准物质进行测定,检测结果为112.10 μg/L,落在其标准值及不确定度范围之内(120.00& #177;15.00)μg/L。 结论 所建立的血铅检测方法各项确认参数符合要求,方法的不确定性在容许范围内。 Abstract:Objective To improve a method on determination of lead in blood by graphite furnace atomic absorption spectrometry and to estimate the uncertainty of the application. Methods Mixed solution containing 0.20% nitric acid and 0.10% Triton X-100 was used as diluent, and mixed solution containing 0.10% palladium nitrate and 0.06% magnesium nitrate was used as matrix modifier. Referring to the recommended instrument measuring conditions, an AA-900T graphite furnace atomic absorption spectrophotometer was applied to determine lead in blood. The limits of detection and the lower limit of quantification of the proposed revised measurement were estimated. The accuracy were determined by national standard reference material. Results The detection limit was 0.84 μg/L and the quantification lower limit was 2.80 μg/L. The linear range was 2.80-200.00 μg/L, and the relative standard deviation was 1.24% for blood lead at 100.0 μg/L. The concentration of the national standard reference material was 112.10 μg/L and within the required uncertainty range of (120.00& #177;15.00) μg/L. Conclusion All parameters of the proposed method meet the requirement of the national clinic laboratory technique guideline. The estimated uncertainty of the method falls within the acceptable range.
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