矽肺患者肺灌洗液巨噬细胞自噬与凋亡分析
Autophagy and Apoptosis of Alveolar Macrophages in Whole Lung Lavage Fluid of Silicosis Patients
-
摘要:
目的 研究不同期别矽肺患者肺灌洗回收液中巨噬细胞自噬活动强弱的变化以及与凋亡的联系。 方法 选择接尘观察对象、壹期、贰期、叁期矽肺患者各30例作为研究对象。调查职业史,并采用大容量全肺灌洗术,收集灌洗回收液,分离肺巨噬细胞。运用透射电子显微镜观察肺组织切片中自噬前体、自噬体、自噬溶酶体等,并检测肺巨噬细胞早期凋亡率。 结果 观察对象肺巨噬细胞胞核周围可观察到自噬体;壹期矽肺患者肺巨噬细胞胞核附近可观察到自噬前体、胞浆中其他细胞器均较少见;贰期矽肺患者肺巨噬细胞胞核周围自噬体、自噬溶酶体较多见;叁期矽肺患者肺巨噬细胞胞浆中可见成群大小不等初级溶酶体,但未观察到典型的自噬前体、自噬体、自噬溶酶体。观察对象、壹期、贰期、叁期矽肺患者肺巨噬细胞凋亡率分别为(15.28& #177;0.20)%、(18.13& #177;0.58)%、(19.56& #177;1.07)%、(23.92& #177;2.52)%,各组间凋亡率差异有统计学意义(P < 0.01)。 结论 不同期别矽肺患者肺巨噬细胞自噬活动强弱以及凋亡程度不同,巨噬细胞自噬与凋亡在矽肺纤维化发生发展中可能起一定作用。 Abstract:Objective To study autophagy activity changes and apoptosis of alveolar macrophages (AMs) in lung lavage fluid retrieved from patients at various stages of silicosis. Methods Observed participants with exposure to silicious dust and silicosis patients at stage I, II, and III (n=30 for each group) were chosen as study subjects. Information of occupational history was collected, and AMs of all subjects were harvested by massive whole lung lavage. The morphologies of autophagosome precursor, autophagosome, and autolysosome were observed under transmission electron microscope. Early apoptosis of AMs was also detected. Results Autophagosomes were found around the nuclear in the AMs of the observed participants. Autophagosome precursors were identified in the AMs of the stage I silicosis patients, but other cytoplasm organelles were rarely seen. Autophagosomes and autolysosomes in the stage II silicosis patients were also found. Primary lysosomes of different sizes were observed in the stage III silicosis patients, but no typical autophagosome precursor, autophagosome, or autolysosome. The AMs apoptosis rates in the observed participants and the stage I, II & III silicosis patients were (15.28& #177;0.20)%, (18.13& #177;0.58)%, (19.56& #177;1.07)%, and (23.92& #177;2.52)% respectively, and the differences among them were statistically significant (P < 0.01). Conclusion There are differences in the autophagic activity and apoptosis of AMs among silicosis patients at different stages. AMs autophagy and apoptosis may play an important role in the development of silicosis.
下载:
