杨正丽, 李倩, 邵一鸣, 赵一帆, 张芬, 肖洪喜, 常秀丽, 张玉彬, 周志俊. 百草枯对小鼠脾脏淋巴细胞的影响[J]. 环境与职业医学, 2019, 36(6): 571-575. DOI: 10.13213/j.cnki.jeom.2019.19081
引用本文: 杨正丽, 李倩, 邵一鸣, 赵一帆, 张芬, 肖洪喜, 常秀丽, 张玉彬, 周志俊. 百草枯对小鼠脾脏淋巴细胞的影响[J]. 环境与职业医学, 2019, 36(6): 571-575. DOI: 10.13213/j.cnki.jeom.2019.19081
YANG Zheng-li, LI Qian, SHAO Yi-ming, ZHAO Yi-fan, ZHANG Fen, XIAO Hong-xi, CHANG Xiu-li, ZHANG Yu-bin, ZHOU Zhi-jun. Effects of paraquat on lymphocytes in spleen of mice[J]. Journal of Environmental and Occupational Medicine, 2019, 36(6): 571-575. DOI: 10.13213/j.cnki.jeom.2019.19081
Citation: YANG Zheng-li, LI Qian, SHAO Yi-ming, ZHAO Yi-fan, ZHANG Fen, XIAO Hong-xi, CHANG Xiu-li, ZHANG Yu-bin, ZHOU Zhi-jun. Effects of paraquat on lymphocytes in spleen of mice[J]. Journal of Environmental and Occupational Medicine, 2019, 36(6): 571-575. DOI: 10.13213/j.cnki.jeom.2019.19081

百草枯对小鼠脾脏淋巴细胞的影响

Effects of paraquat on lymphocytes in spleen of mice

  • 摘要: 背景 百草枯(PQ)是一种广泛使用的除草剂。目前的研究主要针对PQ的神经毒性。然而,PQ对外周免疫系统影响的研究尚不全面。

    目的 观察PQ对外周免疫系统的影响,特别是对淋巴细胞数量、分化及活化状态的影响。

    方法 6~8周龄SPF级雌性C57BL/6小鼠随机分为对照组、2 mg/kg PQ染毒1周组和2周组,每组8只小鼠。采用皮下注射方式染毒,染毒频率为每周一次,分别于染毒1、2周后取脾脏,用流式细胞术检测脾脏细胞和淋巴细胞数量,以及淋巴细胞分化和活化情况。

    结果 与对照组相比,PQ染毒小鼠脾脏细胞的数量在第1周和第2周差异无统计学意义(P > 0.05);CD4+T细胞和CD8+T细胞的数量在第1周和第2周差异亦无统计学意义(P > 0.05);B细胞的数量在第2周时高于对照组(t=-2.304,P < 0.05),在第1周时差异无统计学意义(P > 0.05)。与对照组相比,PQ在第2周时降低辅助性T细胞1(Th1细胞)(t=0.019,P < 0.05)和辅助性T细胞2(Th2细胞)(t=0.038,P < 0.05)占CD4+T细胞的比例,在第1周时无影响(P > 0.05);不影响辅助性T细胞17(Th17细胞)和调节性T细胞(Treg细胞)占CD4+T细胞的比例(P > 0.05)。此外,PQ在第2周时降低B细胞表面主要组织相容性复合体Ⅱ类分子I-A(t=0.047,P < 0.05)和B细胞表面抗原CD40(t=0.000,P < 0.01)的表达,不影响B细胞表面抗原CD86的表达(P > 0.05)。

    结论 PQ可增加B细胞数量,可以抑制CD4+T细胞向Th1和Th2方向分化,并抑制B细胞活化,提示PQ可以通过影响免疫细胞数量、分化和活化而引起免疫功能紊乱。

     

    Abstract: Background Paraquat (PQ) is a widely used herbicide. Current research focuses on its neurotoxicity. However, the impact of PQ on peripheral immune system is not comprehensively studied.

    Objective This study aims to study the effects of PQ on peripheral immune system, especially on the number, differentiation, and activation of lymphocytes.

    Methods SPF female C57BL/6 mice, 6-8 weeks old, were randomly assigned to three groups, namely control group, 2 mg/kg PQ for 1 week group, and for 2 weeks group, with 8 mice in each group. Mice were subcutaneously injected with PQ once a week. Spleen was harvested 1 or 2 weeks after exposure to measure the number of splenocytes and lymphocytes, as well as the differentiation and activation of lymphocytes by flow cytometry.

    Results Compared with the control group, the number of splenocytes did not change after 1 or 2 weeks of PQ exposure (P > 0.05); the numbers of CD4+T cells and CD8+ T cells did not change either (P > 0.05); the number of B cells was higher after 2 weeks of exposure (t=-2.304, P < 0.05), but not after 1 week of exposure (P > 0.05). Compared with the control group, PQ decreased the proportions of type 1 T helper (Th1) cells (t=0.019, P < 0.05) and type 2 T helper (Th2) cells (t=0.038, P < 0.05) to CD4+T cells after 2 weeks of exposure, but not after 1 week of exposure (P > 0.05); PQ did not affect the proportions of T helper 17 (Th17) cells and regulatory T (Treg) cells (P > 0.05) to CD4+T cells. In addition, PQ reduced the expressions of a major histocompatibility complex class Ⅱ molecule I-A (t=0.047, P < 0.05) and B-cell surface antigen CD40 (t=0.000, P < 0.01) after 2 weeks of exposure, but did not affect the expression of B-cell surface antigen CD86 (P > 0.05).

    Conclusion PQ exposure could increase the number of B cells, inhibit the differentiation of CD4+T cells into Th1 and Th2 cells, and inhibit the activation of B cells, suggesting that PQ could cause immune dysfunction by affecting the number, differentiation, and activation of immune cells.

     

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