赵刚, 吴依然, 毛宏晖, 马文彦, 张林, 仇玉兰, 牛侨, 张文平. 苯并[a]芘亚急性染毒后小鼠焦虑样行为及海马神经元的变化[J]. 环境与职业医学, 2018, 35(7): 648-653. DOI: 10.13213/j.cnki.jeom.2018.18190
引用本文: 赵刚, 吴依然, 毛宏晖, 马文彦, 张林, 仇玉兰, 牛侨, 张文平. 苯并[a]芘亚急性染毒后小鼠焦虑样行为及海马神经元的变化[J]. 环境与职业医学, 2018, 35(7): 648-653. DOI: 10.13213/j.cnki.jeom.2018.18190
ZHAO Gang, WU Yi-ran, MAO Hong-hui, MA Wen-yan, ZHANG Lin, QIU Yu-lan, NIU Qiao, ZHANG Wen-ping. Changes of anxiety-like behaviors and hippocampal neurons in mice with subacute benzo[a]pyrene exposure[J]. Journal of Environmental and Occupational Medicine, 2018, 35(7): 648-653. DOI: 10.13213/j.cnki.jeom.2018.18190
Citation: ZHAO Gang, WU Yi-ran, MAO Hong-hui, MA Wen-yan, ZHANG Lin, QIU Yu-lan, NIU Qiao, ZHANG Wen-ping. Changes of anxiety-like behaviors and hippocampal neurons in mice with subacute benzo[a]pyrene exposure[J]. Journal of Environmental and Occupational Medicine, 2018, 35(7): 648-653. DOI: 10.13213/j.cnki.jeom.2018.18190

苯并a芘亚急性染毒后小鼠焦虑样行为及海马神经元的变化

Changes of anxiety-like behaviors and hippocampal neurons in mice with subacute benzoapyrene exposure

  • 摘要: 目的 观察苯并a芘亚急性染毒后小鼠神经行为及海马CA1区神经元树突、树突棘密度的变化。

    方法 48只C57BL/6小鼠随机分为对照组和低、中、高剂量苯并a芘染毒共4组,经腹腔注射分别给予植物油溶剂和苯并a芘0.8、2.0、5.0 mg/kg,隔天一次,连续4周。通过糖水偏爱实验、旷场实验和高架十字迷宫实验观察苯并a芘亚急性染毒后小鼠神经行为学改变;采用高尔基染色观察海马CA1区内神经元树突、树突棘密度的变化。

    结果 在糖水偏爱实验中,各组小鼠糖水偏好百分比(93.71±0.99)%、(89.43±4.10)%、(89.72±1.23)%、(84.04±5.88)%无差异(P > 0.05)。在旷场实验中:高剂量组小鼠在中心区滞留时间(50.34±12.74)s短于对照组(73.94±29.30)s(P < 0.05);中、高剂量组小鼠中心区进入次数(15.88±8.84、13.25±7.92)少于对照组(28.00±13.33),周边区运动距离(9 688.23±1 382.15)、(9 926.83±1 477.06)mm较对照组(7 655.69±2 392.45)mm长(均P < 0.05)。在高架十字迷宫实验中,中、高剂量组小鼠开臂进入次数(5.88±1.96、5.63±2.97)、开臂滞留时间百分比(18.38±5.25)%、(20.90±2.27)%均小于对照组11.63±7.25、(39.80±8.37)%(均P < 0.05)。高尔基染色结果显示高剂量组小鼠海马CA1区神经元树突总长度(1295.88±238.26)μm、树突分支数(16.50±1.38)及树突棘密度(每10μm中的个数)(3.20±0.79)均低于对照组(1732.81±239.69)μm、22.17±2.56、5.90±1.20。

    结论 苯并a芘亚急性暴露可诱发小鼠焦虑样行为,这可能与海马CA1区神经元树突及树突棘可塑性改变相关。

     

    Abstract: Objective To investigate the changes of neurobehavioral performance as well as neuronal dendrites and dendritic spine density in hippocampal CA1 region of mice with subacute benzoapyrene exposure (BaP).

    Methods Forty-eight C57BL/6 mice were randomly divided into four groups receiving either olive oil (control group) or BaP at 0.8, 2.0, and 5.0 mg/kg (low, medium, and high dose groups) every other day for four weeks. The neurobehavioral changes of mice were observed by sucrose preference test, open field test, and elevated plus maze test. The before-and-after-treatment changes of neuronal dendrites and dendritic spine density in hippocampal CA1 region were observed by Golgi staining.

    Results In the sucrose preference test, there was no significant difference in the percentage of sucrose preference among the four groups(93.71±0.99)%, (89.43±4.10)%, (89.72±1.23)%, and (84.04±5.88)%, respectively (P > 0.05). In the open field test, compared with the control groupresidence time, (73.94±29.30) s; frequency of entering central area, 28.00±13.33; distance in surrounding area, (7 655.69±2 392.45) mm, the mice in the high dose group had shorter residence time in central area(50.34±12.74) s, and the mice in the medium dose group and the high dose group showed a less frequency of entering central area (15.88±8.84 and 13.25±7.92) and a longer distance in surrounding area(9 688.23±1 382.15) mm and (9 926.83±1 477.06) mm (Ps < 0.05). In the elevated plus maze test, the frequency of entry into the open arms (5.88±1.96 and 5.63±2.97) and the percentage of time spent in open arms(18.38±5.25)% and (20.90±2.27)% of mice in the medium and high dose groups were smaller than those in the control group11.63±7.25, (39.80±8.37)% (P < 0.05). Golgi staining results showed that the total dendritic length(1 295.88±238.26) μm, number of dendritic branches (16.50±1.38), and dendrite spine density per 10 μm (3.20±0.79) in the hippocampal CA1 neurons of the high dose group were lower than those in the control group(1732.81±239.69)μm, 22.17±2.56, and 5.90±1.20, respectively.

    Conclusion Subacute BaP exposure could induce anxiety-like behavior in mice, which may be related to the change of plasticity of neuron dendrites and dendritic spines in hippocampal CA1 region.

     

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