吴松林, 秦绚, 郭松超. 牛磺酸对染锰大鼠纹状体单胺类神经递质的影响[J]. 环境与职业医学, 2018, 35(8): 756-760. DOI: 10.13213/j.cnki.jeom.2018.18184
引用本文: 吴松林, 秦绚, 郭松超. 牛磺酸对染锰大鼠纹状体单胺类神经递质的影响[J]. 环境与职业医学, 2018, 35(8): 756-760. DOI: 10.13213/j.cnki.jeom.2018.18184
WU Songlin, QIN Xuan, GUO Song-chao. Effects of taurine on striatum monoamine neurotransmitters in manganese exposed rats[J]. Journal of Environmental and Occupational Medicine, 2018, 35(8): 756-760. DOI: 10.13213/j.cnki.jeom.2018.18184
Citation: WU Songlin, QIN Xuan, GUO Song-chao. Effects of taurine on striatum monoamine neurotransmitters in manganese exposed rats[J]. Journal of Environmental and Occupational Medicine, 2018, 35(8): 756-760. DOI: 10.13213/j.cnki.jeom.2018.18184

牛磺酸对染锰大鼠纹状体单胺类神经递质的影响

Effects of taurine on striatum monoamine neurotransmitters in manganese exposed rats

  • 摘要: 目的 探讨不同剂量牛磺酸对锰染毒大鼠纹状体神经递质的影响。

    方法 取SD健康雄性大鼠156只,随机分为对照组,10、15、20 mg/kg 3个剂量的染锰组和9个牛磺酸干预组,牛磺酸干预剂量分别为100、150、200 mg/kg;每组12只大鼠。对照组腹腔注射等量生理盐水;染锰组腹腔注射MnCl2,每天1次,连续12周;牛磺酸干预组与相应剂量的染锰组相同处理染毒,并在每周二、四、六腹腔注射MnCl2后再腹腔注射牛磺酸进行干预,共12周。开颅分离出大鼠纹状体,应用高效液相色谱法测定牛磺酸、多巴胺(DA)和5-羟色胺(5-HT)含量,采用石墨炉原子吸收法和紫外比色法分别测定锰含量和单胺氧化酶(MAO)活性。

    结果 各锰染毒组(2.61±0.73)、(2.75±0.37)、(2.98±0.52)μg/g和牛磺酸干预组(2.52±0.74)、(2.42±0.70)、(2.57±0.40)、(2.56±0.46)、(2.51±0.51)、(2.66±0.75)、(2.82±0.98)、(2.88±0.56)、(2.86±0.91)μg/g大鼠纹状体锰含量均高于对照组(0.60±0.20)μg/g,差异均有统计学意义(均P < 0.05)。20 mg/kg染锰组牛磺酸含量(712.68±128.69)μg/g较对照组减少,差异具有统计学意义(P < 0.05)。染锰组大鼠纹状体DA、5-HT水平DA分别为(6 284.63±1 243.94)、(6 173.54±826.68)、(6 014.21±636.48)μg/g,5-HT分别为(172.22±23.79)、(166.39±42.73)、(164.82±43.43)μg/g均低于对照组(7 814.91±1 284.63)μg/g和(251.34±51.26)μg/g(均P < 0.05);150、200 mg/kg牛磺酸干预10 mg/kg染锰组大鼠纹状体DA和5-HT水平显示增加效果(P < 0.05)。染锰组大鼠纹状体MAO活性分别为(14.65±1.89)、(16.45±1.13)、(17.05±1.86)U(/h · mg)均高于对照组(12.64±1.10)U(/h · mg)(均P < 0.05);各牛磺酸干预组(除100 mg/kg牛磺酸干预10 mg/kg染锰组外)MAO活性均比同剂量染锰组降低(均P < 0.05)。

    结论 锰可致大鼠纹状体部分牛磺酸耗竭,MAO活性增高,DA和5-HT水平降低,牛磺酸在一定程度上对锰诱导的大鼠纹状体神经递质下降有保护作用。

     

    Abstract: Objective To test the effects of taurine on striatum neurotransmitters in manganese exposed rats.

    Methods A total of 156 healthy male SD rats were randomly divided into 1 control group, 3 manganese exposure groups (10, 15, and 20 mg/kg MnCl2, respectively), and 9 taurine intervention groups based on orthogonal design (100, 150, and 200 mg/kg taurine, respectively), with 12 rats in each group. The control group was intraperitoneally injected with equal amount of saline; the manganese exposure groups were intraperitoneally injected with MnCl2, once a day for continuously 12 weeks; the taurine intervention groups were treated with MnCl2 at the same dose as the manganese exposure groups, and intraperitoneally injected with taurine after MnCl2 injection on every Tuesday, Thursday, and Saturday for 12 weeks. All rats were sacrificed and striatums were removed to determine the levels of taurine, dopamine (DA), and 5-hydroxytryptamine (5-HT) by high performance liquid chromatography, the level of manganese by graphite furnace atomic absorption spectrometry, and monoamine oxidase (MAO) activity by UV spectrophotometry.

    Results The average striatum manganese levels of the 3 manganese exposure groups(2.61±0.73), (2.75±0.37), and (2.98±0.52) μg/g, respectively and the 9 taurine intervention groups(2.52±0.74), (2.42±0.70), (2.57±0.40), (2.56±0.46), (2.51±0.51), (2.66±0.75), (2.82±0.98), (2.88±0.56), and (2.86±0.91) μg/g, respectively were higher than that of the control group(0.60±0.20) μg/g (Ps < 0.05). The average taurine level in the group exposed to 20 mg/kg manganese(712.68±128.69) μg/g was lower than that of the control group (P < 0.05). The average DA and 5-HT levels of the 3 manganese exposure groupsDA:(6 284.63±1 243.94), (6 173.54±826.68), and (6 014.21±636.48) μg/g, respectively; 5-HT:(172.22±23.79), (166.39±42.73), and (164.82±43.43)μg/g, respectively were lower than those of the control group(7 814.91±1 284.63)μg/g and (251.34±51.26)μg/g, respectively (Ps < 0.05). The taurine intervention at 150 and 200 mg/kg increased the levels of DA and 5-HT in the group exposed to 10mg/kg manganese (P < 0.05). The striatum monoamine oxidase activities of the 3 manganese exposure groups(14.65±1.89), (16.45±1.13), and (17.05±1.86) U/(h·mg), respectively were higher than that of the control group(12.64±1.10) U/(h·mg) (Ps < 0.05). The striatum monoamine oxidase activities of all the taurine intervention groups (except the group exposed to 10 mg/kg manganese and intervened with 100 mg/kg taurine) were significantly lower than those of the groups exposed to same dose of manganese (Ps < 0.05).

    Conclusion Manganese could reduce the levels of taurine, DA, and 5-HT, and increase the enzyme activity of MAO in rat striatum. Taurine has a protective effect on the decrease of neurotransmitters in striatum of rats induced by manganese.

     

/

返回文章
返回