三丁基锡暴露对斑马鱼体脂的影响
Effect of tributyltin on body fat of zebrafsh
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摘要:
目的 研究三丁基锡对斑马鱼体脂的影响。
方法 采用受精后7 d的AB品系斑马鱼设空白对照、溶剂对照及3个试验组，每组100尾，试验组剂量分别为12.5、25.0、50.0 ng/L三丁基锡，将幼鱼连续染毒至受精后30 d。麻醉后，测量其体质量指数(body mass index，BMI)，检测三酰甘油、总胆固醇水平，并通过HE染色观察其肝脏病理变化，冰冻切片油红O染色观察其肝脏脂肪变化。
结果 25.0、50.0 ng/L三丁基锡组的体重、BMI、三酰甘油水平较空白对照组升P < 0.01)；体重分别为(2.66±0.33)、(3.72±0.58)、(1.16±0.25)mg，BMI分别为(0.07±0.02)、(0.10±0.03)、(0.03±0.01)mg/mm2，三酰甘油水平分别为(24.41±0.80)、(29.47±0.79)、(17.87±0.50)μmol/g(以每g蛋白计)。各剂量组总胆固醇水平与空白对照组间差异无统计学意义。HE染色结果显示25.0、50.0 ng/L剂量组中部分幼鱼肝脂肪变性。50.0 ng/L剂量组中肝组织油红O着色较空白对照组明显加深。
结论 三丁基锡可增加斑马幼鱼脂肪水平，具有致肥胖作用。
Abstract:Objective To study on the effect of tributyltin (TBT) on body fat of zebrafish.
Methods AB zebrafish larvae at 7 days post-fertilization (dpf) were divided into a blank control group, a solvent control group, and three TBT groups, with 100 larvae in each group. The three TBT groups were exposed to 12.5, 25.0, and 50.0 ng/L TBT until 30 dpf, respectively. Then body weight, length, body mass index (BMI), triglyceride (TG), and total cholesterol (TC) of each group were measured or calculated. HE staining and frozen oil red O staining were used to observe liver histological changes and hepatic steatosis of zebrafish samples.
Results The body weight, BMI, and TG in the 25.0 ng/L and 50.0 ng/L TBT groups were significantly higher than those in the blank control group (P < 0.01). The body weights were (2.66±0.33), (3.72±0.58), and (1.16±0.25) mg, respectively; the BMI were (0.07±0.02), (0.10±0.03), and (0.03±0.01)mg/mm2, respectively; the TG levels were (24.41±0.80), (29.47±0.79), and (17.87±0.50) μmol/g (per g of protein), respectively. No difference in TC was found between the TBT groups and the blank control group. Hepatic steatosis was observed in the samples of the 25.0ng/L and 50.0ng/L TBT groups. Deeper liver oil red O staining in the 50.0ng/L group was observed compared with the blank control group.
Conclusion TBT could increase the fat level of zebrafish, showing an obesogenic effect.
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