李金成, 纪敏涛, 王帅, 盛志杰, 李孟阳, 李晓印, 张翔, 李冰燕, 张增利. 镉染毒对糖尿病小鼠胫骨影响的Micro-CT研究[J]. 环境与职业医学, 2018, 35(6): 531-535. DOI: 10.13213/j.cnki.jeom.2018.17626
引用本文: 李金成, 纪敏涛, 王帅, 盛志杰, 李孟阳, 李晓印, 张翔, 李冰燕, 张增利. 镉染毒对糖尿病小鼠胫骨影响的Micro-CT研究[J]. 环境与职业医学, 2018, 35(6): 531-535. DOI: 10.13213/j.cnki.jeom.2018.17626
LI Jin-cheng, JI Min-tao, WANG Shuai, SHENG Zhi-jie, LI Meng-yang, LI Xiao-yin, ZHANG Xiang, LI Bing-yan, ZHANG Zeng-li. Micro-CT study on tibia of diabetic mice post cadmium exposure[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 531-535. DOI: 10.13213/j.cnki.jeom.2018.17626
Citation: LI Jin-cheng, JI Min-tao, WANG Shuai, SHENG Zhi-jie, LI Meng-yang, LI Xiao-yin, ZHANG Xiang, LI Bing-yan, ZHANG Zeng-li. Micro-CT study on tibia of diabetic mice post cadmium exposure[J]. Journal of Environmental and Occupational Medicine, 2018, 35(6): 531-535. DOI: 10.13213/j.cnki.jeom.2018.17626

镉染毒对糖尿病小鼠胫骨影响的Micro-CT研究

Micro-CT study on tibia of diabetic mice post cadmium exposure

  • 摘要: 目的 应用Micro-CT技术探讨糖尿病模型联合镉染毒对小鼠胫骨的影响。

    方法 40只SPF级8周龄雄性C57BL6小鼠,随机分为对照组、氯化镉染毒组、糖尿病模型组、糖尿病模型联合氯化镉染毒组(以下简称为联合组)。氯化镉染毒组以3.5 mg/kg氯化镉腹腔注射染毒,每周3次,连续4周;糖尿病模型组以40 mg/kg链脲佐菌素腹腔注射,连续5 d,注射后1~2周内检测,以空腹血糖≥ 11.1 mmol/L为成模标准;联合组在糖尿病模型成模后进行染毒,操作同前;对照组以同等体积生理盐水腹腔注射。实验结束后,测量小鼠剥离软组织后的胫骨湿重和骨长度,取右侧胫骨进行Micro-CT扫描并进行三维重建,检测骨密度、骨体积分数、骨表面积体积比、骨小梁厚度、骨小梁数目、骨小梁分离度等指标。

    结果 各组小鼠每100 g体重的胫骨湿重及骨长度差异无统计学意义(P > 0.05)。形态学观察结果显示:与对照组相比,氯化镉染毒组、糖尿病模型组、联合组胫骨松质骨骨小梁数目减少,骨小梁厚度降低,排列疏松;与糖尿病模型组相比,联合组变化更加明显。与对照组(0.160±0.029)g/cm3、(14.781±4.754)%、(13.397±2.962)mm-1、(2.964±0.777)mm-1、(0.118±0.013)mm相比,氯化镉染毒组(0.123±0.013)g/cm3、(7.972±2.404)%、(8.533±2.221)mm-1、(1.787±0.513)mm-1、(0.147±0.015)mm、联合组(0.121±0.008)g/cm3、(8.571±1.710)%、(8.902±1.065)mm-1、(1.840±0.280)mm-1、(0.154±0.008)mm小鼠胫骨松质骨骨密度、骨体积分数、骨表面积体积比、骨小梁数目降低,骨小梁分离度增加(均P < 0.05);与糖尿病模型组相比,联合组上述指标差异亦有统计学意义(均P < 0.05)。各组小鼠胫骨皮质骨骨密度差异没有统计学意义(P > 0.05)。与对照组(5.973±0.418)mm-1相比,糖尿病模型组(6.876±0.469)mm-1及联合组(7.568±0.595)mm-1小鼠胫骨皮质骨骨表面积体积比增加(P < 0.05);与氯化镉染毒组(32.765±4.053)%、(6.038±0.579)mm-1相比,联合组(39.901±3.386)%、(7.568±0.595)mm-1皮质骨骨体积分数和骨表面积体积比增加(P < 0.05)。

    结论 镉染毒可降低糖尿病小鼠胫骨松质骨骨体积分数、骨表面积体积比、骨小梁数目、骨密度,增加骨小梁分离度,说明镉染毒可导致糖尿病小鼠胫骨骨质疏松。

     

    Abstract: Objective To investigate the effect of diabetic model mice combined with cadmium exposure on tibia by Micro-CT imaging.

    Methods Forty SPF eight-week-old male C57BL6 mice were randomly divided into four groups:control group, cadmium chloride group, diabetes mellitus model group, and diabetes mellitus model combined with cadmium chloride group (combined exposure group). The cadmium chloride group was intraperitoneally injected with 3.5 mg/kg cadmium chloride 3 times per week for 4 weeks. The diabetes mellitus model was intraperitoneally injected with 40 mg/kg streptozotocin 5 days per week and detected for fasting plasma glucose after 1-2 weeks, and fasting plasma glucose ≥ 11.1 mmol/L indicated that the model was established. The diabetes mellitus model group was treated with cadmium chloride as previously described to establish a combined exposure group. The control group was intraperitoneally injected with normal saline at the same volume. After the treatment protocol, bone wet weight/body weight and bone length were measured after soft tissues were stripped from bone; right tibia of the mice was scanned by Micro-CT for three-dimensional reconstruction, and the measurements included bone mineral density, bone volume fraction, bone surface/bone volume, trabecular thickness, trabecular number, and trabecular separation.

    Results No differences in bone wet weight per 100 g body weight and bone length were found among the four groups (P > 0.05). The morphological observation results showed that, compared with the control group, the other three groups had decreased trabecular number and trabecular thickness together with loose structure, and compared with the diabetes mellitus model group, the combined exposure group had more obvious such changes. Compared with the control group(0.160±0.029) g/cm3, (14.781±4.754)%, (13.397±2.962) mm-1, (2.964±0.777) mm-1, and (0.118±0.013) mm, the bone mineral density, bone volume fraction, bone surface/bone volume, and trabecular number were decreased while trabecular separation of tibial cancellous bone were increased in the cadmium chloride group(0.123±0.013) g/cm3, (7.972±2.404)%, (8.533±2.221) mm-1, (1.787±0.513) mm-1, and (0.147±0.015) mm and the combined exposure group(0.121±0.008) g/cm3, (8.571±1.710)%, (8.902±1.065) mm-1, (1.840±0.280) mm-1, and (0.154±0.008) mm (Ps < 0.05); compared with the diabetes mellitus model group, all the above indicators were significanthy different in the combined exposure group (Ps < 0.05). However, there was no difference in the bone mineral density of tibial cortical bone among the four groups (P > 0.05). Compared with the control group(5.973±0.418) mm-1, the diabetes mellitus model(6.876±0.469) mm-1 and the combined exposure group(7.568±0.595) mm-1 showed increased bone surface/bone volume of tibial cortical bone (P < 0.05); compared with the cadmium chloride group(32.765±4.053)% and (6.038±0.579)mm-1, the combined exposure group(39.901±3.386)% and (7.568±0.595)mm-1 showed increased bone volume fraction and bone surface/bone volume of tibial cortical bone (P < 0.05).

    Conclusion Cadmium exposure could reduce bone volume fraction, bone surface/bone volume, trabecular number, and bone mineral density, and increase trabecular separation of tibial cancellous bone of diabetic mice, indicating osteoporosis of tibial cancellous bone.

     

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